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• 方法与技术 • 上一篇    

响应面法优化枯草芽孢杆菌NHS1产芽孢发酵培养

胡瑞萍1,2,丁贤1,2*,李来好1,李俊伟1,伍文超2,徐宁2   

  1. (1中国水产科学研究院南海水产研究所, 农业部水产品加工重点实验室, 广州 510300;2广州市海威水产科技有限公司, 广州 510300)
  • 出版日期:2018-02-10 发布日期:2018-02-10

Optimization of fermentation medium composition by response surface methodology for the spore production of Bacillus subtilis.

HU Rui-ping1,2, DING Xian1,2*, LI Lai-hao1, LI Jun-wei1, WU Wen-chao2, XU Ning2   

  1. (1Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2Guangzhou Haiwei Aquatic Biotechnology Co. Ltd, Guangzhou 510300, China).
  • Online:2018-02-10 Published:2018-02-10

摘要: 为提高枯草芽孢杆菌(Bacillus subtilis)NHS1菌株发酵液中芽孢含量,采用单因素试验与响应曲面法优化试验相结合的方式,对该菌发酵培养基中的碳源、氮源以及无机盐等组成成分进行了优化。通过单因素试验、Plackett-Burman试验、最陡爬坡试验以及Box-Bchnken试验构建响应方程,得到方程为:Y=-0.30+3.853X1+0.0928X2+0.623X3-0.913X1×X1-0.00704X2×X2-0.0433X3×X3-0.0033X1×X2-0.0700X1×X3+0.00167X2×X3。利用该方程预测得到最优培养基:淀粉1.88 g·L-1、NaCl 6.83 g·L-1、玉米粉5.60 g·L-1,酵母粉10 g·L-1,蛋白胨10 g·L-1,牛肉膏15 g·L-1,葡萄糖2 g·L-1,MgSO43- g·L-1。利用优化培养基,在36 ℃、170 r·min-1条件下摇瓶发酵72 h,芽孢数达到2.42×109 cfu·mL-1,比优化前提高1.5倍。

关键词: HYDRUS-1D模型, 渭北旱塬, 土壤水分, 深剖面

Abstract: Both one factor at atime method and response surface methodology were used toenhance the spore content ofBacillus subtilis NHS1 in fermentation broth. A response surfaceregression equation was established based on the results of onefactor at atime, PlackettBurman, steepest ascent and Box-Behnken experimental designs. The optimal mediumformulation, as predicted from the equation, consisted of 1.88 g·L-1 starch, 6.83 g·L-1 NaCl, 5.60 g·L-1 corn flour, 10 g·L-1 yeast extract powder, 10 g·L-1 peptone, 15 g·L-1 beefextract, 2 g·L-1 glucose, 3 g·L-1 MgSO4. The yield of the spore reached 2.42×109cfu·mL-1, which was 1.5fold higher than that before the optimization by using the optimized medium at36 ℃ with shaking at a speed of 170 r·min-1 for 72 h.

Key words: deep profiles, HYDRUS-1D model, Weibei rainfed highland, soil moisture