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生态学杂志 ›› 2010, Vol. 29 ›› Issue (06): 1240-1244.

• 研究简报 • 上一篇    下一篇

莠去津慢性染毒对河蟹血淋巴细胞DNA的影响

穆淑梅;康现江**;张志甫;郭明申;李凤超   

  1. 河北大学生命科学学院,河北保定 071002
  • 出版日期:2010-06-10 发布日期:2010-06-10

Impacts of atrazine chronic poisoning on hemolymph DNA of Eriocheir sinensis.

MU Shu-mei;KANG Xian-jiang;ZHANG Zhi-fu;GUO Ming-shen;LI Feng-chao   

  1. College of Life Sciences, Hebei University, Baoding 071002, Hebei, China
  • Online:2010-06-10 Published:2010-06-10

摘要: 利用不同浓度(0.01、0.1、1 mg·L-1)的莠去津对河蟹进行慢性染毒处理90 d,采用单细胞凝胶电泳技术检测其对河蟹血淋巴细胞DNA损伤的影响。结果表明,不同浓度的莠去津能引起河蟹血淋巴细胞的DNA损伤,且随着浓度的增加,TM值、TailDNA逐渐变大,尤以1 mg·L-1浓度组最为显著。证明一定浓度的莠去津可以造成河蟹血淋巴细胞DNA链的断裂,破坏其DNA构。

关键词: 中间锦鸡儿根瘤菌, 多样性, 抗逆性

Abstract: Atrazine is one of the most widely used herbicides in China. In this paper, Eriocheir sinensis was treated with 0.01, 0.1, and 1 mg·L-1 of atrazine for 90 days, and single-cell gel electrophoresis was used to study the impacts of atrazine chronic poisoning on the hemolymph DNA of E. sinensis. The fluorescence microscope observation and the CASP analysis of the images showed that all the three concentrations of atrazine could induced the DNA damage of E. sinensis hemolymph. With the increase of atrazine concentration, the TM value and the percentage of TailDNA increased gradually, and the impacts were most significant when the concentration of atrazine was 1 mg·L-1, suggesting that definite concentration atrazine could induce the broken of DNA chain, and destroy the DNA structure of E. sinensis hemolymph.

Key words: Caragana intermedia Rhizobia, Diversity, Resistance