汞、豆磺隆复合污染对土壤脲酶活性及动力学特征的影响

生态学杂志

汞、豆磺隆复合污染对土壤脲酶活性及动力学特征的影响

杨春璐¹;孙铁珩²;和文祥³

¹辽宁大学环境学院，沈阳 110036；²沈阳大学环境工程重点实验室，沈阳 110044；³西北农林科技大学资源环境学院， 陕西杨凌 712100

收稿日期:2007-08-27 修回日期:1900-01-01 出版日期:2008-05-10 发布日期:2008-05-10

Effects of mercury and chlorimuron-ethyl on soil urease activity and its kinetic characteristics.

YANG Chun-lu¹;SUN Tie-hang²;HE Wen-xiang³

¹College of Environmental Science, Liaoning University, Shenyang 110036
, China; ²Key Laboratory of Environmental Engineering, Shenyang University, Shenyang 110044, China;³College of Resources and Environment,
Northwest A&F University, Yangling 712100, Shaanxi, China

Received:2007-08-27 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10

摘要/Abstract

摘要： 通过室内模拟研究了重金属汞(Hg)和除草剂豆磺隆单一和复合污染条件下对草甸棕壤和黑土4个土样脲酶活性和动力学参数的影响。结果发现：汞对脲酶活性、K_m、V_max、V_max/K_m均有负效应，其影响幅度分别为39%～98%、46%～74%、90%～98%、20%～68%；HgCl₂浓度与脲酶活性之间符合对数方程，与3动力学参数之间呈显著(P<0.05)或极显著(P<0.01)线性负相关；豆磺隆对脲酶有激活作用，除2号土样脲酶活性对不同浓度豆磺隆影响差异不明显外，其余3土样最大增幅分别为17%、18%和15%，原因可能是豆磺隆的加入提高了脲酶酶促反应的初速度；汞、豆磺隆复合污染对脲酶活性和3特征参数均产生负效应，其影响幅度与汞单一污染情况相似，但与污染物浓度之间的相关性较差，豆磺隆的加入使汞与脲酶之间的相互作用趋于复杂。

关键词: 铅, 杂草, 养分, 丛枝菌根真菌(AMF)

Abstract: With incubation test, this paper studied the effects of mercury (Hg), chlorimuronethyl, and their combination on the urease activity and its kinetic characteristics in meadow brown soil and black soil. The results showed that g had negative effects on the activity,K_m,V_max, and V_max/K_m of soil urease, with the affecting range of 39%-98%, 46%-74%, 90%-98%, and 20%-68%, respectively. The relationship between soil urease activity and HgCl₂ concentration could be described with logarithmic equation, and the K_m, V_max, and V_max/K_m of soil urease were linearly negatively correlated with HgCl₂ concentration. Chlorimuron-ethyl could activate soil urease activity, possibly because of the enhancement of initial velocity of urease reaction. The combination of Hg and chlorimuron-ethyl also had negative effects on the urease activity and its kinetic characteristics, with the affecting range similar to that of Hg, but poorer correlations were observed with the pollutants concentration. The addition of chlorimuron-ethyl made the interaction between Hg and soil urease more complicated.

Key words: Lead, Weed, Nutrient, Vesicular-arbuscular mycorrhizae

杨春璐;孙铁珩;和文祥 . 汞、豆磺隆复合污染对土壤脲酶活性及动力学特征的影响[J]. 生态学杂志.

YANG Chun-lu¹;SUN Tie-hang²;HE Wen-xiang³. Effects of mercury and chlorimuron-ethyl on soil urease activity and its kinetic characteristics. [J]. cje.

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