To investigate the responses of soil N₂O emission to temperature under straw and biochar addition, soil N₂O emission of CK (without straw and biochar), LC (1% biochar), HC (2% biochar) and HS (2.75% straw) was examined at three incubation temperatures of 10 ℃ (T1), 20 ℃ (T2) and 30 ℃ (T3), respectively. The results showed that the concentration of NH₄⁺-N in all the four treatments decreased and the concentration of NO₃^--N increased with increasing incubation temperature. At the same temperature, the concentration of NH₄⁺-N in soil under additions of different materials was in an order of CK>LC>HC>HS, and the concentration of NO₃^--N was HC>LC>CK>HS. At different temperatures, soil N₂O emission under additions of different materials was significantly different. With rising temperature (T1-T3), the cumulative soil N₂O emission under CK, LC, HC and HS treatments increased by 46.49%-412.81%, 64.69%-456.55%, 7.42%-145.96%, and 105.91%-1421.66%, respectively. At T3, the more biochar added, the more obvious inhibition effect on N₂O emission. The cumulative N₂O emission of LC and HC treatments was significantly lower than that of CK, with a reduction of 64.27% and 159.43%, respectively. The addition of straw significantly promoted soil N₂O emission, with the cumulative N₂O emission of HS treatment being 178.12%, 456.87% and 721.54% higher than that of CK, LC and HC, respectively. Therefore, rising temperature is beneficial to soil N₂O emissions, and returning straw to the field promotes soil N₂O emissions. Returning straw-derived biochar to the field significantly reduces soil N₂O emissions.