钙磷对蜈蚣草淹水胁迫响应的调控效应

doi:10.13292/j.1000-4890.202405.010

摘要/Abstract

摘要： 蜈蚣草（Pteris vittata）是世界上第一个被发现的砷超积累植物，是砷污染土壤植物修复的理想材料。淹水胁迫是影响蜈蚣草生长和分布的重要环境因子，限制其在高水分环境中的应用。研究蜈蚣草对淹水胁迫的响应及其调控措施具有重要的现实意义。钙（Ca）和磷（P）可提高植物对干旱、低温、盐碱等逆境胁迫的抗性，促进其生存和生长。然而，关于Ca、P对植物淹水胁迫响应及其调控效应的研究较少。本研究以蜈蚣草为对象，分析饱和持水量（相对含水率35%）和淹水胁迫（相对含水率40%）对叶片光合速率、超氧化物歧化酶（SOD）活性、细胞膜脂过氧化产物丙二醛（MDA）含量及生物量的影响，以不添加Ca、P为对照（CK），研究Ca、P对蜈蚣草淹水胁迫抗逆性的调控效应。结果表明：相较于35%含水率，40%含水率淹水胁迫使叶片光合速率降低30.8%，SOD活性降低71.7%，MDA含量提高46.4%，生物量降低77.3%，表明淹水胁迫使蜈蚣草抗氧化胁迫能力降低，使叶片光合速率和生物量均显著下降（P<0.05）；淹水胁迫时，添加5～20 g·kg^-1 Ca和0.1～0.2 g·kg^-1 P使叶片光合速率、SOD活性和生物量较CK分别提高0.15～1.65、0.58～1.91和0.91～5.01倍，MDA含量降低14%～36.1%，表明Ca、P可缓解淹水胁迫对蜈蚣草的不利影响，且缓解作用随Ca、P添加量的升高而增强；相关性分析证实，添加Ca、P显著降低含水率与叶片光合速率、SOD活性、生物量的负相关性，及含水率与MDA的正相关性，且该趋势随Ca、P添加量的升高而增强，表明添加Ca、P使蜈蚣草对淹水胁迫的抗逆性提高。因此，Ca、P可通过提高抗氧化酶活性，增强活性氧清除能力，减轻膜脂过氧化，从而维持较高的光合活性，进而提高蜈蚣草对淹水胁迫的耐受性。

关键词: 蜈蚣草, 淹水胁迫, 钙磷, 光合速率, 酶活性, 生物量

Abstract: Pteris vittata is the first reported arsenic (As) hyperaccumulator, which is an ideal plant for remediation of As contaminated soils. Flooding stress is an important factor affecting the growth and geographical distribution of P. vittata, limiting its application in environment with high humidity. Therefore, it is of great importance to understand the response of P. vittata to flooding stress and the underlying mechanisms. Calcium (Ca) and phosphorus (P) can promote the survival and growth of plants by improving their resistance to drought, cold, salt and alkali stresses. The effects of Ca and P on plant responses to flooding stress are less studied. We analyzed photosynthetic rate, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and biomass of P. vittata under saturation water retention (relative moisture content 35%) and flooding stress (relative moisture content 40%). The regulatory effects of Ca and P on P. vittata flooding stress resistance were studied with no Ca or P as a control (CK). Compared to the treatment with 35% moisture content, leaf photosynthetic rate decreased by 30.8%, SOD activity decreased by 71.7%, MDA content increased by 46.4%, and biomass decreased by 77.3% under 40% flooding stress, indicating that flooding stress reduced the resistance of P. vittata to antioxidant stress, resulting in significantly decreased leaf photosynthetic rate and biomass (P<0.05). Under flooding stress, 5-20 g·kg^-1 Ca or 0.1-0.2 g·kg^-1 P increased photosynthetic rate, SOD activity, and biomass by 0.15-1.65, 0.58-1.91, and 0.91-5.01 times, and decreased MDA content by 14%-36.1% compared to CK. This result indicated that Ca and P addition can alleviate the adverse effects of flooding stress on P. vittata, which was enhanced with increasing Ca and P application amounts. The correlation analysis confirmed that Ca and P application reduced the negative correlation between moisture content and leaf photosynthetic rate, SOD activity, biomass, and the positive correlation between moisture content and MDA, which was increased with increasing Ca and P application, indicating that Ca and P can improve the resistance ability of P. vittata to flooding stress. Therefore, Ca and P application can improve the tolerance of P. vittata to flooding stress via increasing antioxidant enzymes activity, increasing scavenging active oxygen species, and reducing membrane lipid peroxidation, thereby maintaining high photosynthetic activities.

Key words: Pteris vittata, flooding stress, calcium and phosphorus, photosynthetic rate, enzyme activity, biomass

徐其静, 毛佳璇, 马露冉, 杨晓莉, 刘雪. 钙磷对蜈蚣草淹水胁迫响应的调控效应[J]. 生态学杂志, 2024, 43(5): 1271-1278.

XU Qijing, MAO Jiaxuan, MA Luran, YANG Xiaoli, LIU Xue. Regulation of calcium and phosphorus on the response of Pteris vittata to flooding stress.[J]. Chinese Journal of Ecology, 2024, 43(5): 1271-1278.

[1]	许红丽, 王树强, 张玉兰, 李守中, 尹云锋. 菌糠源生物堆肥对黄瓜种植土壤微生物活性、数量和多样性的影响 [J]. 生态学杂志, 2024, 43(5): 1279-1290.
[2]	付瑞桐, 李德军, 胡培雷, 段鹏鹏, 张玉玲. 岩性对森林土壤有机碳矿化的影响 [J]. 生态学杂志, 2024, 43(4): 1000-1007.
[3]	张国微, 薛建辉, 马洁, 王翰琨. 喀斯特退化山地不同类型人工林土壤养分与酶活性 [J]. 生态学杂志, 2024, 43(3): 616-622.
[4]	张斌, 吕玉峰, 李利, 冯美臣, 王超, 宋晓彦, 杨武德, 张美俊. 丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响 [J]. 生态学杂志, 2024, 43(3): 644-655.
[5]	李义林, 李坤, 李建查, 潘志贤, 史亮涛, 刘小刚, 方海东, 岳学文. 有机肥和套种对干热区火龙果土壤微生物特性和产量、品质的影响 [J]. 生态学杂志, 2024, 43(3): 656-664.
[6]	李炳怡, 刘冠宏, 顾泽, 刘晓东, 舒立福. 火干扰对油松林土壤细菌群落的影响 [J]. 生态学杂志, 2023, 42(6): 1355-1364.
[7]	何婉莹, 谷思玉, 谷邵臣, 车延静, 王紫颖, 袁望博, 杨佳宇. 深翻-旋耕轮耕与有机肥配施对农田黑土团聚体内酶活性的影响 [J]. 生态学杂志, 2023, 42(5): 1090-1098.
[8]	张加涛, 张雅荣, 刘亚玲, 索明春, 徐柱文. 不同恢复措施对内蒙古乌拉特中旗荒漠草原植物群落的影响 [J]. 生态学杂志, 2023, 42(4): 828-837.
[9]	宋鹏, 杨振中, 万祖梁, 王鑫彤, 张文忠, 王国骄. 水稻秸秆秋季湿耙还田对土壤酶活性和养分的影响 [J]. 生态学杂志, 2023, 42(3): 569-576.
[10]	王杰, 李雪英, 陈启沂, 张扬, 唐保军. 长期饥饿下熊本牡蛎生理代谢和酶活性变化 [J]. 生态学杂志, 2023, 42(1): 146-151.
[11]	胡可, 严光荣, 唐安军. 非生物因子对东方香蒲种群有性更新的影响 [J]. 生态学杂志, 2022, 41(6): 1121-1127.
[12]	王广昊, 孔星杰, 孙彩丽, 吴攀. 铅锌废渣场周边土地利用方式转变对土壤胞外酶活性的影响 [J]. 生态学杂志, 2022, 41(6): 1166-1172.
[13]	李晗, 吕刚, 李叶鑫, 程昊天, 刘禹鑫. 露天煤矿排土场不同植被恢复模式土壤养分和酶活性的差异性 [J]. 生态学杂志, 2022, 41(5): 919-924.
[14]	赵婧, 段磊磊, 王铭, 王升忠, 陈聪, 李鸿凯, 李兴丽. 水文管理措施对长白山区恢复泥炭地土壤酶活性的影响 [J]. 生态学杂志, 2022, 41(5): 948-954.
[15]	张亚坤, 杨凯, 苗晨, 徐爽, 张金鑫. 辽东山区不同树种幼苗根际土微生物性质及其对氮磷添加的响应 [J]. 生态学杂志, 2022, 41(4): 633-640.

钙磷对蜈蚣草淹水胁迫响应的调控效应

Regulation of calcium and phosphorus on the response of Pteris vittata to flooding stress.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价