长期饥饿下熊本牡蛎生理代谢和酶活性变化

doi:10.13292/j.1000-4890.202301.007

生态学杂志 ›› 2023, Vol. 42 ›› Issue (1): 146-151.doi: 10.13292/j.1000-4890.202301.007

长期饥饿下熊本牡蛎生理代谢和酶活性变化

王杰^1,2,李雪英²,陈启沂^1,2,张扬^1,2,唐保军^2*

（¹上海海洋大学水产与生命学院，上海 201306； ²中国水产科学研究院东海水产研究所，上海 200090）

出版日期:2023-01-10 发布日期:2023-02-01

Changes of physiological metabolism and enzyme activities of Crassostrea sikamea under long-term starvation.

WANG Jie^1,2, LI Xue-ying², CHEN Qi-yi^1,2, ZHANG Yang^1,2, TANG Bao-jun^2*

(¹College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; ²East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China).

Online:2023-01-10 Published:2023-02-01

摘要/Abstract

摘要： 为了探讨熊本牡蛎（Crassostrea sikamea）在长期饥饿胁迫下的生理代谢响应，测定了长期饥饿（0～80 d）以及再投喂（90～118 d）状态下熊本牡蛎的耗氧率、排氨率以及肝胰腺组织乳酸脱氢酶、琥珀酸脱氢酶、丙酮酸激酶、超氧化物歧化酶、碱性磷酸酶和溶菌酶的活性。结果表明：长期饥饿下，熊本牡蛎的耗氧率和排氨率极显著降低（P<0.01）；随着饥饿时间的延长，熊本牡蛎乳酸脱氢酶、琥珀酸脱氢酶、丙酮酸激酶、超氧化物歧化酶和碱性磷酸酶活性均呈现波动下降的趋势，溶菌酶活性呈现波动上升的趋势；再投喂后熊本牡蛎的耗氧率、排氨率均极显著升高（P<0.01）；长期饥饿对熊本牡蛎代谢和免疫功能造成损伤，熊本牡蛎通过降低代谢率和代谢酶的活性应对长期饥饿胁迫。本研究可为海水贝类饥饿胁迫下代谢调节机制的研究提供参考。

关键词: 熊本牡蛎, 饥饿胁迫, 耗氧率, 排氨率, 酶活性

Abstract: To clarify the physiological and metabolic responses of Crassostrea sikamea under long-term starvation stress, we measured oxygen consumption rate, ammonia excretion rate and activities of lactate dehydrogenase, succinate dehydrogenase, pyruvate kinase, superoxide dismutase, alkaline phosphatase and lysozyme in hepatopancreas after long-term starvation (0-80 d) and refeeding (90-118 d). The results showed that oxygen consumption and ammonia excretion rate of C. sikamea decreased significantly under long-term starvation (P<0.01). With the prolongation of starvation, the activities of lactate dehydrogenase, succinate dehydrogenase, pyruvate kinase, superoxide dismutase, and alkaline phosphatase in C. sikamea showed a trend of fluctuating decline, while lysozyme activity showed a trend of fluctuating rise. After refeeding, oxygen consumption and ammonia excretion rate of C. sikamea significantly increased (P<0.01). Long-term starvation may damage the metabolism and immune functions of C. sikamea, and C. sikamea, and they respond to long-term starvation stress by reducing metabolic rate and metabolic enzyme activities. Our results provide a reference for the study of metabolic regulation mechanism of marine shellfish under starvation stress.

Key words: Crassostrea sikamea, starvation stress, oxygen consumption rate, ammonia excretion rate, enzymatic activity.

王杰, 李雪英, 陈启沂, 张扬, 唐保军. 长期饥饿下熊本牡蛎生理代谢和酶活性变化[J]. 生态学杂志, 2023, 42(1): 146-151.

WANG Jie, LI Xue-ying, CHEN Qi-yi, ZHANG Yang, TANG Bao-jun. Changes of physiological metabolism and enzyme activities of Crassostrea sikamea under long-term starvation.[J]. Chinese Journal of Ecology, 2023, 42(1): 146-151.

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长期饥饿下熊本牡蛎生理代谢和酶活性变化

Changes of physiological metabolism and enzyme activities of Crassostrea sikamea under long-term starvation.

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