We investigated the effects of urease inhibitorN(n-butyl) thiophosphoric triamide (NBPT), nitrification inhibitor 2-chloro-6-(trichloromethyl) pyridine (CP), and their combined application on NH₃ volatilization rate, NH₃ accumulation and dynamics of yellow clayey soil in an incubation experiment. Results showed that NH₃ volatilization rate peaked after three days of urea application and then leveled off. CP treatments with different application doses promoted soil NH₃ volatilization and increased the peak value of NH₃ volatilization rate by 10.9%. NBPT treatments with different application doses delayed the peak time of soil NH₃ volatilization and significantly reduced the peak value of NH₃ volatilization rate by 35.0%. NBPT significantly inhibited soil NH₃ volatilization. On the 24th day after incubation, soil NH₃ volatilization amount was reduced by 37.2% in NBPT treatments as compared with urea treatment, while it was increased by 2.1% in CP treatments. Compared with application of CP alone, combined application of CP with NBPT showed synergistic inhibition effect on NH₃ loss. NBPT+CP treatments effectively delayed urea hydrolysis in early time, and thus reduced NH₃ loss by 14.2% (day 24) as compared with urea treatment. However, NH₃ volatilization increased with the increases of CP dose. Furthermore, the dynamics of the cumulative amount of NH₃ volatilization could be quantitativelydescribed by Elovich equation (q_t=a+blnt). Additionally, CP treatments increased the slopeb value, which increased with the increasing dose. NBPT treatments reduced the slope b value, which decreased with the increasing dose. NBPT+CP treatments reduced the slopeb value. Soil NH₃ volatilization rate was significantly positively correlated with soil pH. In conclusion, application ofNBPT or combined with CP could significantly reduce soil NH₃ volatilization rate and soil N loss, and thus improve fertilizer utilization in yellow clayey soil.