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遮荫和种源对青钱柳三萜类化合物积累的影响

邓波1,2,刘桂华1,余云云1,徐丹丹1,叶青青1,方升佐2*#br#   

  1. 1安徽农业大学林学与园林学院, 合肥 230022;2南京林业大学林学院, 南京 210037)
  • 出版日期:2018-02-10 发布日期:2018-02-10

Effects of shading and provenance on triterpenoid accumulation in leaves ofCyclocarya paliurus.

DENG Bo1,2, LIU Gui-hua1, YU Yun-yun1, XU Dan-dan1, YE Qing-qing1, FANG Sheng-zuo2*   

  1. (1School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230022, China; 2College of Forestry, Nanjing Forestry University, Nanjing 210037, China).
  • Online:2018-02-10 Published:2018-02-10

摘要:

青钱柳(Cyclocarya paliurus)是中国特有的药食同源珍稀植物,其叶中含有的活性物质(如三萜)对人体健康具有促进和改善作用。本文以两根一杆青钱柳苗为试材,选择3个种源(B1:五峰;B2:沅陵;B3:沐川)和设置3个遮荫强度(A1:100%光照;A2:30%光照;A3:12%光照),开展遮荫和种源对青钱柳三萜积累和单株三萜产量的影响。结果表明:遮荫处理显著影响了环境中的光量子通量密度、空气温度和相对湿度,明显降低了青钱柳生物量的积累,生物量积累最大的处理为全光下的沐川种源(A1B3)。遮荫和种源的交互作用显著影响了三萜单体的含量;五峰(A1)和沅陵(A2)种源中青钱柳酸B和阿江榄仁酸的含量随遮荫强度加强先下降后上升,在透光率为12%时(两层遮荫)达到最大值,平均为1.84和4.17 mg·g-1;而沐川(A3)种源呈直线上升,二者变异范围分别为0.85~2.47和1.88~4.28 mg·g-1;除A2外,A1和A3中的青钱柳甙I的含量随光照减弱而不断下降,变异范围分别为0.20~0.27和1.33~2.52 mg·g-1;另外,遮荫、种源及二者的交互作用显著影响了青钱柳中总三萜和三萜单体的单株产量,其中,总三萜和青钱柳甙I单株产量最高的处理为A1B3,分别为2523.4和208.5 mg·株-1;而青钱柳酸B和阿江榄仁酸单株产量最高的处理为A2B3;总三萜和三萜单体的最低单株产量均在两层遮荫下获得。因此,种源的选择与栽培条件的优化对提高药用青钱柳单位面积三萜产量具有重要意义。
 

关键词: 耕地, 灰色关联分析, 潜性酸, GIS

Abstract: Cyclocarya paliurus, a multiple functional plant, has traditionally been used for drug formulations or ingredients of functional foods in China. Previous studies showed that the leaves of C. paliurus contain many physiologically active compounds (mainly triterpenoids) which were known to perform important physiological activity in human body. In the present study, a splitplot randomized design was used to examine the effects of shading (three levels) and provenance (three provenances) on triterpenoid content and triterpenoid accumulation over a growing season. Three shading levels were subjected to three light intensities, including 100% solar radiation (A1, without shading net), 30% of full solar radiation (A2, covered with one layer of shading net at 2 m height), and 12% of full solar radiation (A3, covered with two layers of shading net at 2 m height). Three provenances were Wufeng (B1) from Hubei Province, Yuanling (B2) from Hunan Province, and Muchuang (B3) from Sichuan Province. Our results showed that shading significantly affected the photosynthetic photon flux density, air temperature, and relative humidity, and thus significantly decreased the biomass accumulation. The highest biomass was detected in treatment A1B3. The interaction of shading and provenance significantly influenced the triterpenoid contents in leaves ofC. paliurus. The contents of cyclocaric acid B and arjunolic acid in provenances B1 and B2 were decreased in 30% light transmittance (A2) but increased in 12% light transmittance (A3). The highest contents of cyclocaric acid B and arjunolic acid were detected in 12% light transmittance. For provenance B3, however, the contents of cyclocaric acid B and arjunolic acid were positively correlated with increasing shading level. In addition, except for A2, the contents of cyclocarioside I in provenances B1 and B3 were decreased with thedeclined light intensity, with the variations ranging 0.20-0.27 mg·g-1 and 1.33-2.52 mg·g-1, respectively. Both the main and interactive effects of shading and provenance had significant influence on triterpenoid yield per plant. The highest yield of total triterpenoid and cyclocarioside I was detected in treatment A1B3, with the mean values of 2523.4 and 208.5 mg per plantrespectively, while the highest yields of cyclocaric acid B and arjunolic acid were found intreatment A2B3. The lowest yields of total and three individual triterpenoids were detected in A3 treatment. Overall, our results suggest a complex relationship between light environment,genotype and triterpenoid production, and highlight that selecting suitable provenance ofC. paliurus and optimizing cultivation system would be crucial for a high triterpenoid production.

Key words: cropland, grey relational analysis, potential acid, GIS