辣椒疫病拮抗菌的分离、鉴定及其生防效果

生态学杂志

辣椒疫病拮抗菌的分离、鉴定及其生防效果

谈泰猛¹,黎继烈^1*,申爱荣^1,3,严准²,张梦君¹,祝久香¹,曾粮斌^2*,赵梦瑞^1#br#

（¹ 中南林业科技大学，长沙 410004； ² 中国农业科学院麻类研究所，长沙 410205； ^3 湖南省林业科学院，长沙 410004）

出版日期:2017-04-10 发布日期:2017-04-10

Isolation, identification and biocontrol effect of antagonistic bacteria on Phytophthora capsici.

TAN Tai-meng¹, LI Ji-lie^1*, SHEN Ai-rong^1,3, YAN Zhun², ZHANG Meng-jun¹, ZHU Jiu-xiang¹, ZENG Liang-bin^2*, ZHAO Meng-rui^1#br#

(¹Central South University of Forestry and Technology, Changsha 410004, China; ²Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; ^3Hunan Academy of Forestry, Changsha 410004, China).

Online:2017-04-10 Published:2017-04-10

摘要/Abstract

摘要： 从健康辣椒植株的根际土壤中分离筛选对辣椒疫病有较强抑制作用的拮抗菌，为其生防利用奠定基础。采用稀释平板涂布法和对峙培养法进行拮抗菌的分离筛选，根据菌株形态学特征、生理生化特性以及16S rDNA序列分析对其进行鉴定，通过盆栽试验检测其生防效果。结果表明：从土样中分离筛选到一株对辣椒疫病具有显著拮抗作用的细菌IBFCBF-4，IBFCBF-4对另外8种植物病原菌也有显著抑制作用；综合其形态学特征、生理生化特性以及16S rDNA序列信息，确定其为枯草芽孢杆菌(Bacillus subtilis)；盆栽试验表明，IBFCBF-4对辣椒疫病防治效果为64.28%，相对于对照组，地上部分鲜重增加45.2%，根鲜重增加34.3%；IBFCBF4对辣椒疫病防治及促生效果显著，具有良好地开发和利用价值。

关键词: 温度, 草甸湿地, 水分, 净生态系统碳交换量, 科尔沁

Abstract: Bacteria were isolated and screened from the rhizosphere soil of healthy pepper to obtain antagonistic strains with strong inhibitory effect on Phytophthora capsici. This study aimed to provide a basis for utilization of biocontrol strains. Antagonistic strains were screened out by spread plate and dual culture method. Strains were identified by morphological, physiological and biochemical characteristics, and 16S rDNA sequences analysis. The biocontrol potential was determined by pot experiment. IBFCBF-4 was isolated from the soils, which showed strong inhibitory effect on P. capsici. And it also had strong antagonistic effect against other eight kinds of plant pathogens. This bacterium was identified as Bacillus subtilis by methods described above. Pot experiment showed the control efficiency was 64.28% on P. capsici, and the fresh weight of shoot and root increased by 45.2% and 34.3%, respectively, when compared with the control group. IBFCBF-4 had a strong biocontrol effect on P. capsici and a significant growthpromoting effect on pepper; it was of great development and utilization value for P. capsici control in the field.

Key words: net ecosystem CO₂ exchange, meadow wetland, moisture, Horqin, temperature

谈泰猛1,黎继烈1*,申爱荣1,3,严准2,张梦君1,祝久香1,曾粮斌2*,赵梦瑞1. 辣椒疫病拮抗菌的分离、鉴定及其生防效果[J]. 生态学杂志.

TAN Tai-meng1, LI Ji-lie1*, SHEN Ai-rong1,3, YAN Zhun2, ZHANG Meng-jun1, ZHU Jiu-xiang1, ZENG Liang-bin2*, ZHAO Meng-rui1. Isolation, identification and biocontrol effect of antagonistic bacteria on Phytophthora capsici.[J]. cje.

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