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外源ATP对盐胁迫下油菜幼苗生长的影响

赖晶,李巧丽,张小花,粱娟红,张腾国*   

  1. (西北师范大学生命科学学院, 兰州 730070)
  • 出版日期:2020-06-10 发布日期:2020-06-10

Effects of exogenous ATP on the growth of Brassica campestris seedlings under salt stress.

LAI Jing, LI Qiao-li, ZHANG Xiao-hua, LIANG Juan-hong, ZHANG Teng-guo*   

  1. (College of Life Sciences, Northwest Normal University, Lanzhou 730070, China).
  • Online:2020-06-10 Published:2020-06-10

摘要: 研究了外源ATP处理对盐胁迫下油菜幼苗生长的影响,探讨了过氧化氢(H2O2)和钙离子(Ca2+)作为信号分子在ATP对油菜幼苗耐盐性调控过程中的作用。结果表明:与单独NaCl处理相比,ATP+NaCl处理降低了油菜幼苗死细胞数量、ROS(O2-.和H2O2)含量、离子(Ca2+、Na+、Cl-)含量、MDA含量及Na+/K+比和相对电导率,增加了叶片中叶绿素、脯氨酸、可溶性糖含量和抗氧化酶(SOD、POD、CAT、APX)活性,提高了抗氧化酶基因(CATSODAPXGR)、NADPH氧化酶基因(RBOHD、RBOHF)、P5CS1基因、MAPK激酶基因(MAPK3、MAPK6)、耐盐基因(NHX1、SOS1)转录;与ATP+NaCl处理相比,ATP+NaCl+抑制剂(DPI、DMTU和EGTA)处理下油菜幼苗中相对电导率、MDA、叶绿素、脯氨酸、可溶性糖含量和抗氧化酶(SOD、POD、CAT、APX)活性及上述基因表达量均呈不同程度降低,表明外源ATP可提高NaCl胁迫下油菜叶片细胞活性、ROS含量、离子含量、叶绿素含量、渗透调节物质、抗氧化酶活性及相关基因的表达量,缓解膜质损伤。此外,H2O2和Ca2+信号分子也参与了ATP增强油菜幼苗耐盐性过程的调控。

关键词: 生态特征, 党参, 最大熵模型, 生态适宜区

Abstract: We examined the effects of exogenous ATP treatment on the growth of Brassica campestris seedlings under salt stress, and clarified the roles of signal molecules, hydrogen peroxide (H2O2) and calcium ion (Ca2+) in the ATP regulation of salt tolerance. The results showed that, compared with the single NaCl treatment, ATP+NaCl treatment decreased the number of dead cells, ROS (O2-· and H2O2) content, ion (Ca2+, Na+, Cl-) content, MDA content, Na+/K+ ratio, and relative electrical conductivity. The chlorophyll, proline, soluble sugar content, antioxidant enzyme (SOD, POD, CAT, APX) activity, transcriptional regulation of antioxidant enzyme genes (CAT, SOD, APX, GR), NADPH oxidase genes (RBOHD, RBOHF), P5CS1 gene, MAPK kinase genes (MAPK3, MAPK6), and salt tolerance genes (NHX1, SOS1) were all elevated. Compared with ATP+NaCl treatment, the relative electrical conductivity, MDA, chlorophyll, proline, soluble sugar, the activities of antioxidant enzymes (SOD, POD, CAT, APX), and the expression of the above genes were decreased when treated with ATP+NaCl+inhibitors (DPI, DMTU and EGTA). These results indicated that exogenous ATP application could increase cell activity, ROS content, ion content, chlorophyll content, osmotic regulator, antioxidant enzyme activity and related gene expression in B. campestris under salt stress. In addition, signal molecules of H2O2 and Ca2+ were involved in the ATP regulation of enhancing salt tolerance in B. campestris.

Key words: ecological suitable region, ecological characteristics., maximum entropy model, Codonopsis pilosula