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克百威和毒死蜱农药降解菌的筛选与鉴定

王新**,陆佳靓,刘丽,崔丽,梁吉艳   

  1. (沈阳工业大学理学院, 沈阳 110870)
  • 出版日期:2014-03-10 发布日期:2014-03-10

Screening and identification of bacteria for degradation of pesticides carbofuran and chlorpyrifos.

WANG Xin**, LU Jia-liang, LIU Li, CUI Li, LIANG Ji-yan   

  1. (School of Science, Shenyang University of Technology, Shenyang 110870, China)
  • Online:2014-03-10 Published:2014-03-10

摘要:

采用多株细菌对克百威和毒死蜱农药分别进行降解实验,得到了具有优势的降解细菌并对其进行鉴定。结果表明,LLBK2、LLBK10对100 mg·L-1克百威的降解效果最好,48 h内的降解率分别为58.0%和60.0%,72 h内的降解率分别为82.8%和83.8%。LLBD2、LLBD4对100 mg·L-1毒死蜱的降解效果最好,48 h内的降解率分别为70.6%和61.5%,72 h内的降解率分别为92.3%和91.4%。同时对这4株细菌进一步鉴定,LLBK2、LLBK10、LLBD2均为蜡状芽胞杆菌(Bacillus circus),其16S rDNA基因序列在GenBank登录号分别为:JQ917438、HQ833023、JX847612,LLBD4为芽孢杆菌(Bacillus),其16S rDNA基因序列在GenBank登录号为JX010962。
 

关键词: 种群, 栖息地, 竹林, 相关性分析, 潜在栖息地

Abstract: Several strains of bacteria were applied in the degradation experiments of carbofuran and chlorpyrifos, and the preponderant bacteria were identified by comparison. The LLBK2 and LLBK10 showed the best degradation effect on the 100 mg·L-1 of carbofuran, with the degradation rates of 58.0% and 60.0% in 48 hours, and 82.8% and 83.8% in the 72 hours, respectively. The LLBD2 and LLBD4 showed the best degradation effect on the 100 mg·L-1 of chlorpyrifos, with the degradation rates of 70.6% and 61.5% in the 48 hours, and 92.3% and 91.4% in 72 hours, respectively. Through further microbiological identification, LLBK2, LLBK10, LLBD2 could be classified as Bacillus circus. The accessing numbers of their 16S rDNA gene sequences in the GenBank were JQ917438, HQ833023, and JX847612, respectively. LLBD4 could be categorized as Bacillus, which has the GenBank accessing number of JX010962.

Key words: potential habitat, habitat, bamboo forest, population, correlation analysis.