关键词: 山杨, 雌雄异株, 生长速率, 气候响应

Abstract: In order to understand the toxicity effect of compound fertilizer on aquatic organisms, the erythrocytes of Bufo raddei tadpoles at Gosner stages 31, 42 and 46 were taken as the test materials to observe their micronuclei and nuclear anomalies under effects of compound fertilizer. When exposed to the compound fertilizer at concentrations of 1, 1.5, 2, 2.5 and 3 g·L^-1, two types of micronuclei, a big one and a small one, appeared in the erythrocytes of the tadpoles, and in the nuclear anomalies, serious nuclear lobulation and double-nuclei appeared. The frequencies of appearing micronuclei and nuclear anomalies were obviously lower at stage 42 than at stages 31 and 46. Among the five experimental groups, the group exposed to 2.5 g·L^-1 of compound fertilizer had no significant differences in the micronucleus, double-nucleus, and nuclear lobulation with the control group. The results suggested that the tadpoles at their different development stages had obvious difference in resisting the adverse factors in habitats, and no obvious dose-effect correlation existed between the micronuclei and nuclear anomalies and the concentration of compound fertilizer.

Key words: Populus davidiana, dioecy, growth rate, response to climate.