欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志 ›› 2021, Vol. 40 ›› Issue (10): 3205-3213.doi: 10.13292/j.1000-4890.202110.026

• 研究报告 • 上一篇    下一篇

基于骨龄学方法鉴定大别山商城肥鲵的年龄

陈玉翠,陈锦云*   

  1. (淮南师范学院, 资源与环境生物技术安徽普通高校重点实验室, 安徽淮南 232038)
  • 出版日期:2021-10-10 发布日期:2022-04-01

Identification of the age of Pachyhynobius shangchengensis of Dabie Mountains based on bone age method.

CHEN Yu-cui, CHEN Jin-yun*   

  1. (Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes, Huainan Normal University, Huainan 232038, Anhui, China).
  • Online:2021-10-10 Published:2022-04-01

摘要: 2014年4月—2018年8月,在大别山鹞落坪国家级自然保护区内采集50只中国特有物种商城肥鲵(Pachyhynobius shangchengensis)个体,采用常规石蜡切片法对其肱骨、股骨、指骨和趾骨各骨节等骨质材料进行年龄鉴定并比较其年轮特征,根据体长分布频率数据初步确定其年龄为参照,比较肱骨、股骨、指骨和趾骨各骨节在年龄鉴定读数中的精确性和准确性,以确定适合的年龄鉴定材料。同时以剪切脚趾作为标记,根据野外重捕获得的硬骨组织材料进行骨龄鉴定,验证年龄鉴定的准确性和年龄组划分的合理性。结果表明:趾骨的总精确度和总准确性均最高,股骨次之,肱骨最小;小于8龄的个体,骨切片年轮线清晰,骨龄学法适用于该物种的年龄鉴定,分析认为后肢第三、第四趾第2骨节是鉴定商城肥鲵年龄的最佳材料。通过分析野外商城肥鲵的生长规律发现,年龄与体重、体长和头长具有显著相关性,相关系数分别为0.975、0.945和0.97,8龄后这种相关性有降低趋势。

关键词: 商城肥倪, 年龄鉴定, 生长速率, 骨龄学, 生活史

Abstract: We collected 50 individuals ofPachyhynobius shangchengensis, an endemic species in China, in Yaoluoping National Nature Reserve of Dabie Mountains from April 2014 to August 2018. We determined the ages of humerus, femur, phalanxes of finger, and phalanxes of foot by conventional paraffin section method, and compared their growth ring characteristics. According to the frequency data of body length distribution, the age was primarily determined as the reference, and then the precision and accuracy of humerus, femur, phalanxes of finger, and phalanxes of foot were compared in order to determine the suitable age identification materials. The toe was cut as a marker, and the bone age was identified according to the hard bone tissue materials obtained from field recapture to verify the accuracy of age identification and the rationality of age group division. The results showed that the total precision and accuracy of phalanxes of foot were the highest, followed by femur and humerus. For the individuals younger than 8 years old, the ring line of bone section was clear, and the bone age method was suitable for age identification. The second bone node of the third and fourth toe of hind limb was the best material for age identification. There was a significant correlation between age and body weight, body length and head length, with respective correlation coefficient of 0.975, 0.945 and 0.97. However, such correlation tended to  decrease for individuals older than 8 years.

Key words: Pachyhynobius shangchengensis, age determination, growth rate, skeletochronology, life history.