关键词: 玉米秸秆, 低温, 复配微生物, 降解特性

Abstract: The slow decomposition of stover returned to field in winter leads to low emergence rate of seedlings and aggravation of diseases and insect pests in northern China. Here, we explored the degradation characteristics of corn stover by exogenous rot promoting microorganisms under low temperature, with the aim to provide scientific support for field application of low-temperature corn stover degradation compound bacteria. Achromobacter deleyi (A3), Pseudomonas plecoglossicida(A4),Aspergillus terreus(A), andPhanerochaete chrysosporium(P) were used to build composite microbial system GF-Z. The results showed that degradation rate of corn stover by the composite microbial system GF-Z under 15 ℃ cultivated for 30 d was 38.79%. The activities of filter paper enzyme (FPA), xylanase and laccase were 14.59 U·mL^-1, 5.86 U·mL^-1 and 54.49 U·mL^-1, respectively. Under the conditions of salt concentration 0.02%-2.5% and initial pH 4.0-9.0, the activities of FPA, xylanase and laccase of GF-Z were maintained above 81%, and the degradation rate of corn stover was 36.2%-37.5%. When temperature ranged from 13 to 25 ℃, the activities of FPA, xylanase and laccase of GF-Z were maintained above 85%, and the degradation rate of corn stover reached 38.79%.

Key words: corn stover, low temperature, composite microbial system, decomposition characteristics.