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生态学杂志 ›› 2023, Vol. 42 ›› Issue (12): 3066-3072.doi: 10.13292/j.1000-4890.202312.006

• 技术与方法 • 上一篇    

耐低温菌株Pseudomonas sp. W4筛选及其异养-好氧脱氮性能

包红旭1,胡家伟1,2,任宏鹏1,2,韩斯琴2,3,张颖2,3,王泳璇4,史荣久2,3*


  

  1. 1辽宁大学环境学院, 沈阳 110036; 2中国科学院沈阳应用生态研究所, 沈阳 110016; 3中国科学院污染生态与环境工程重点实验室, 沈阳 110016; 4沈阳环境科学研究院辽宁省城市生态重点实验室, 沈阳 110167)

  • 出版日期:2023-12-10 发布日期:2024-06-10

Isolation and nitrogen removal capability of a psychrotolerant bacterium, Pseudomonas sp. strain W4.

BAO Hongxu1, HU Jiawei1,2, REN Hongpeng1,2, HAN Siqin2,3, ZHANG Ying2,3, WANG Yongxuan4, SHI Rongjiu2,3*#br#

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  1. (1College of Environmental Science, Liaoning University, Shenyang 110036, China; 2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 3Key Laboratory of Pollution Ecology and Environmental Engineering, Chinese Academy of Sciences, Shenyang 110016, China; 4Shenyang Academy of Environmental Sciences, Shenyang 110167, China).

  • Online:2023-12-10 Published:2024-06-10

摘要:

从活性污泥中筛选到一株高效的耐低温脱氮菌株W4,评估了该菌株在5、15和25 ℃下的脱氮性能。结果表明,W4属于假单胞菌属(Pseudomonas),能够在4~42 ℃、盐度(NaCl)为0~30 g·L-1与pH 6~9条件下生长。在5 ℃条件下,菌株W4能够在72 h内将初始浓度为76.0 mg·L-1的NH4+-N去除74.3%,相应的脱氮平均速率达0.78 mg·L-1·h-1。好氧条件下菌株在5 ℃时可去除42.4%的NO3--N(86.3 mg·L-1)或38.2%的NO2--N(82.2 mg·L-1);在15、25 ℃条件下菌株脱氮能力进一步提高,对NH4+-N的去除率超过95%,对NO3--N和NO2--N的去除率分别超过66%和69%。菌株W4具有异养硝化好氧反硝化(HA-ND)代谢活性,在硝化过程中不出现NO2--N的积累,在中低温条件下去除无机氮污染具有较好潜力。本研究结果有助于丰富低温脱氮微生物资源并可为HAND脱氮工艺提供支撑。


关键词: 异养硝化, 好氧反硝化, 耐低温, 脱氮

Abstract: A psychrotolerant bacterium strain W4 was isolated from active sludge and its nitrogen-removal capability at 5, 15, and 25 ℃ was evaluated. Results showed that W4 is affiliated to Pseudomonas, and could grow at 4 to 42 ℃, 0 to 30 g·L-1 NaCl, and pH 6 to 9. At 5 ℃, strain W4 removed 74.3% of NH4+-N (initial concentration of 76.0 mg·L-1) within 72 h, corresponding to an average nitrogen-removal rate of 0.78 mg·L-1·h-1. Under aerobic conditions, up to 42.4% of nitrate (86.3 mg·L-1) or 38.2% of nitrite (82.2 mg·L-1) could be removed by strain W4. At 15 and 25 ℃, the nitrogen-removal efficiency was further improved, as evidenced by that more than 95% of ammonium, 66% of nitrate, and 69% of nitrite were removed. Strain W4 showed the activity of heterotrophic nitrification and aerobic denitrification (HA-ND). No nitrite accumulation was observed during nitrification process. The HA-ND capability by strain W4 justifies the potential in the treatment of inorganic nitrogen pollution under meso to psychrophilic conditions. Our findings help enrich psychrotolerant nitrogen-removing microbial resources, and contribute to the nitrogen pollution treatments through HA-ND process.


Key words: heterotrophic nitrification, aerobic denitrification, psychrotolerant, nitrogen removal.