氮添加和采脂对湿地松林土壤酶活性及酶化学计量比的影响

doi:10.13292/j.1000-4890.202201.020

摘要/Abstract

摘要： 大气氮沉降和采脂会引起树木生长和代谢的变化，从而影响土壤养分循环和酶活性。土壤酶和酶化学计量可以揭示土壤碳、氮循环和微生物生长代谢过程的养分限制，但目前亚热带湿地松人工林土壤酶和酶化学计量对氮添加和采脂的响应还不清楚。以亚热带北缘的湿地松人工林为研究对象，设置采脂（resin tapping, RT）和未采脂（no resin tapping, NRT）样地，开展不同强度氮添加（对照0 kg N·hm^-2·a^-1、低氮50 kg N·hm^-2·a^-1、高氮100 kg N·hm^-2·a^-1）试验，探讨氮添加和采脂对湿地松人工林土壤酶活性和酶化学计量比的影响。结果表明：采脂显著降低土壤pH、全磷（TP）含量和微生物生物量碳（MBC），显著增加了土壤可溶性有机碳（DOC）含量；氮添加显著降低了TP、DOC含量；氮添加和采脂对土壤DON和NH₄⁺-N含量具有交互影响作用；采脂显著降低土壤过氧化氢酶(CAT)活性和土壤蔗糖酶/酸性磷酸酶（E_C/P)值，显著提高了土壤酸性磷酸酶（ACP）活性；高氮添加显著降低了未采脂林分土壤脲酶（URE）活性，低氮添加显著提高了采脂林分土壤CAT活性。未采脂林分中，氮添加显著降低土壤E_N/P，高氮添加显著增加E_C/N；RDA分析表明，土壤TP和DOC含量是土壤酶活性及酶化学计量比的主要影响因子。总之，氮添加加剧了土壤微生物的碳、磷限制，氮添加和采脂改变了湿地松人工林土壤碳、氮、磷循环。

关键词: 氮添加, 采脂, 酶活性, 酶化学计量比, 湿地松人工林

Abstract: Atmospheric nitrogen deposition and resin tapping alter tree growth and metabolism, with consequences on soil nutrient cycling and enzyme activities. Soil enzymes and enzymatic stoichiometry can reveal soil carbon and nitrogen cycling and the nutrient limitation of microbial growth and metabolic processes. However, the responses of soil enzymes and enzymatic stoichiometry to nitrogen addition and resin tapping of slash pine (Pinus elliottii) plantation in subtropical area are still unclear. In this study, a field experiment was conducted to examine the effects of three nitrogen addition levels (CK: 0 kg N·hm^-2·a^-1; LN: 50 kg N·hm^-2·a^-1; HN: 100 kg N·hm^-2·a^-1) and resin tapping (RT, resin tapping; NRT, no resin tapping) on the activities of soil hydrolytic enzyme, oxidat6ion enzyme, and their stoichiometry in a slash pine plantation. Resin tapping significantly decreased soil pH, total phosphorus (TP) content and microbial biomass carbon (MBC), while significantly increased dissolved organic carbon (DOC) content. Nitrogen addition significantly reduced soil TP and DOC contents. Nitrogen addition and RT interactively affected the contents of dissolved organic nitrogen (DON) and ammonium nitrogen (NH₄⁺-N). Resin tapping significantly decreased the activity of soil catalase (CAT) and the ratio of soil sucrase/acid phosphatase (E_C/P), but significantly increased the activity of soil acid phosphatase (ACP). HN addition significantly suppressed soil urease (URE) activity in NRT stands, while LN addition significantly promoted CAT activity in RT stands. In the NRT stands, N addition significantly decreased soil E_N/P, while HN addition significantly increased soil E_C/N. Results of redundancy analysis showed that changes in soil enzyme activity and enzymatic stoichiometry were mainly driven by the variations of soil TP and DOC content under N addition. In conclusion, N addition exacerbated soil microbial C and P limitation, and N addition and resin tapping changed soil C, N and P cycling in slash pine plantations.

Key words: nitrogen addition, resin tapping, enzyme activity, enzymatic stoichiometry, slash pine plantation.

刘西军, 蔡天培, 杜杰, 袁莉芹, 陈杰, 徐小牛. 氮添加和采脂对湿地松林土壤酶活性及酶化学计量比的影响[J]. 生态学杂志, 2022, 41(2): 227-235.

LIU Xi-jun, CAI Tian-pei, DU Jie, YUAN Li-qin, CHEN Jie, XU Xiao-niu. Effects of nitrogen addition and resin tapping on soil enzyme activities and their stoichiometry in a slash pine plantation.[J]. Chinese Journal of Ecology, 2022, 41(2): 227-235.

[1]	付瑞桐, 李德军, 胡培雷, 段鹏鹏, 张玉玲. 岩性对森林土壤有机碳矿化的影响 [J]. 生态学杂志, 2024, 43(4): 1000-1007.
[2]	张国微, 薛建辉, 马洁, 王翰琨. 喀斯特退化山地不同类型人工林土壤养分与酶活性 [J]. 生态学杂志, 2024, 43(3): 616-622.
[3]	张斌, 吕玉峰, 李利, 冯美臣, 王超, 宋晓彦, 杨武德, 张美俊. 丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响 [J]. 生态学杂志, 2024, 43(3): 644-655.
[4]	李义林, 李坤, 李建查, 潘志贤, 史亮涛, 刘小刚, 方海东, 岳学文. 有机肥和套种对干热区火龙果土壤微生物特性和产量、品质的影响 [J]. 生态学杂志, 2024, 43(3): 656-664.
[5]	冯蒙蒙, 林永新, 贺子洋, 樊剑波, 贺纪正. 土壤含水量和氮添加对旱地红壤氨氧化微生物功能基因丰度的影响 [J]. 生态学杂志, 2024, 43(2): 480-486.
[6]	李炳怡, 刘冠宏, 顾泽, 刘晓东, 舒立福. 火干扰对油松林土壤细菌群落的影响 [J]. 生态学杂志, 2023, 42(6): 1355-1364.
[7]	何婉莹, 谷思玉, 谷邵臣, 车延静, 王紫颖, 袁望博, 杨佳宇. 深翻-旋耕轮耕与有机肥配施对农田黑土团聚体内酶活性的影响 [J]. 生态学杂志, 2023, 42(5): 1090-1098.
[8]	宋鹏, 杨振中, 万祖梁, 王鑫彤, 张文忠, 王国骄. 水稻秸秆秋季湿耙还田对土壤酶活性和养分的影响 [J]. 生态学杂志, 2023, 42(3): 569-576.
[9]	王斌, 王汝振, 李甜, 张玉革, 姜勇. 大气沉降氮在土壤-植物系统中的留存机制 [J]. 生态学杂志, 2023, 42(2): 463-470.
[10]	王杰, 李雪英, 陈启沂, 张扬, 唐保军. 长期饥饿下熊本牡蛎生理代谢和酶活性变化 [J]. 生态学杂志, 2023, 42(1): 146-151.
[11]	张晓曦, 周雯星, 王丽洁, 刘楚波, 米皓皓. 模拟氮沉降对凋落物早期混合分解中相互作用的影响 [J]. 生态学杂志, 2022, 41(9): 1708-1716.
[12]	王睿照, 毛沂新, 云丽丽, 尤文忠, 张慧东. 氮添加对蒙古栎叶片碳氮磷化学计量与非结构性碳水化合物的影响 [J]. 生态学杂志, 2022, 41(7): 1369-1377.
[13]	王广昊, 孔星杰, 孙彩丽, 吴攀. 铅锌废渣场周边土地利用方式转变对土壤胞外酶活性的影响 [J]. 生态学杂志, 2022, 41(6): 1166-1172.
[14]	高钿惠, 王卫锋, 尚佳州, 李涛, 王升级. 中金杨幼苗叶功能性状与碳氮分配对氮添加的响应 [J]. 生态学杂志, 2022, 41(5): 841-848.
[15]	李晗, 吕刚, 李叶鑫, 程昊天, 刘禹鑫. 露天煤矿排土场不同植被恢复模式土壤养分和酶活性的差异性 [J]. 生态学杂志, 2022, 41(5): 919-924.

氮添加和采脂对湿地松林土壤酶活性及酶化学计量比的影响

Effects of nitrogen addition and resin tapping on soil enzyme activities and their stoichiometry in a slash pine plantation.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价