不同时期喷施NaHS对盐碱胁迫下裸燕麦叶片渗透调节物质和抗氧化活性的影响

doi:10.13292/j.1000-4890.202111.027

摘要/Abstract

摘要： 为确定盐碱条件下裸燕麦的硫化氢（H₂S）最佳施用时期和适宜浓度，采用盆栽土培试验，分别于裸燕麦幼苗期、拔节期、抽穗期、开花期和灌浆期喷施H₂S供体硫氢化钠（NaHS）溶液（0、25、50、100、200和400 μmol·L^-1），研究H₂S对盐碱混合(NaCl∶Na₂SO₄∶NaHCO₃∶Na₂CO₃摩尔比为12∶8∶9∶1)胁迫下裸燕麦叶片渗透调节物质含量和抗氧化活性的影响。结果表明：喷施时期、NaHS浓度及二者的交互作用显著促进或抑制盐碱胁迫下裸燕麦叶片渗透调节物质含量和抗氧化活性；与同一时期不喷施NaHS相比，幼苗期喷施50和200 μmol·L^-1 NaHS分别显著提高ABTS·⁺清除活性和FRAP抗氧化活性，喷施400 μmol·L^-1 NaHS提高总酚含量、O₂^-_·清除活性和总还原活性的效果最好；拔节期喷施50、100和400 μmol·L^-1 NaHS分别显著提高可溶性蛋白、类黄酮和脯氨酸含量30.43%、31.44%和258.59%；抽穗期喷施50、100和400 μmol·L^-1 NaHS分别显著提高原花色素、可溶性糖和游离氨基酸含量150.57%、42.71%和197.93%；开花期喷施100 μmol·L^-1 NaHS的DPPH·清除活性显著提高93.98%，增幅最大；灌浆期喷施400和25 μmol·L^-1 NaHS分别提高K⁺含量和K⁺/Na⁺的作用最显著；喷施时期和NaHS浓度交互作用对裸燕麦叶片有机酸含量和·OH清除活性无显著作用或表现为负效应。采用隶属函数分析综合评价不同时期喷施NaHS对裸燕麦叶片渗透调节和抗氧化活性影响，结果显示，拔节期和抽穗期喷施400 μmol·L^-1 NaHS的综合评价值最大。表明外源H₂S能够通过提高渗透调节和抗氧化活性缓解裸燕麦盐碱胁迫，以拔节抽穗期喷施400 μmol·L^-1 NaHS效果最佳。

关键词: 硫化氢, 裸燕麦, 盐碱胁迫, 渗透调节物质, 抗氧化活性

Abstract: In order to clarify the optimal application period and appropriate concentration of hydrogen sulfide (H₂S), we sprayed H₂S donor sodium hydrosulfide (NaHS) solution (0, 25, 50, 100, 200 and 400 μmol·L^-1) at seedling, jointing, heading, flowering and filling stages of soil potted naked oats to examine the effects of H₂S on the content of osmotic adjustment substance and the antioxidant activity in leaves under salt-alkali mixed stress (NaCl, Na₂SO₄, NaHCO₃, Na₂CO₃ molar ratio is 12∶8∶9∶1). The results showed that spraying period, NaHS concentration, and their interaction significantly promoted or inhibited the content of osmotic adjustment substance and the antioxidant activity. Compared with non-spraying NaHS, spraying 50 and 200 μmol·L^-1 NaHS at the seedling stage significantly increased the scavenging activity of ABTS·⁺ and the antioxidant activity of FRAP; and spraying 400 μmol·L^-1 NaHS performed best in increasing the total phenol content, scavenging activity of O₂^-_· and total reducing activity. Spraying 50, 100, and 400 μmol·L^-1 NaHS at the jointing stage significantly increased the content of soluble protein, flavonoids, and proline by 30.43%, 31.44%, and 258.59%, respectively. Spraying 50, 100, and 400 μmol·L^-1 NaHS at the heading stage significantly increased the content of proanthocyanidins, soluble sugars and free amino acids by 150.57%, 42.71%, and 197.93%, respectively. Spraying 100 μmol·L^-1 NaHS at the flowering stage significantly increased the scavenging activity of DPPH· by 93.98%, with the greatest magnitude of enhancement. Spraying 400 and 25 μmol·L^-1 NaHS at the grouting period had the strongest effect on increasing the K⁺ content and K⁺/Na⁺. The interaction between spraying period and NaHS concentration had no significant or negative effect on organic acid content and the scavenging activity of ·OH in leaves. Using membership function analysis to comprehensively evaluate the effects of spraying NaHS at different periods on the osmotic adjustment and antioxidant activity of naked oat leaves showed that the comprehensive evaluation value of spraying 400 μmol·L^-1 NaHS at the jointing and heading stages was significantly higher than that of other concentrations at other periods. These results indicated that exogenous H₂S can alleviate the saltalkali stress of naked oat by improving osmotic regulation and antioxidant activity, with the best performance of spraying 400 μmol·L^-1 NaHS at the jointing-heading stage.

Key words: hydrogen sulfide, naked oat, saline-alkali stress, osmotic adjustment substance, antioxidant activity.

刘建新, 刘瑞瑞, 刘秀丽, 贾海燕, 卜婷, 李娜. 不同时期喷施NaHS对盐碱胁迫下裸燕麦叶片渗透调节物质和抗氧化活性的影响[J]. 生态学杂志, 2021, 40(11): 3620-3632.

LIU Jian-xin, LIU Rui-rui, LIU Xiu-li, JIA Hai-yan, BU Ting, LI Na. Effects of spraying NaHS at different growth stages on osmotic adjustment substance and antioxidant activity in leaves of naked oat under salinealkali stress.[J]. Chinese Journal of Ecology, 2021, 40(11): 3620-3632.

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不同时期喷施NaHS对盐碱胁迫下裸燕麦叶片渗透调节物质和抗氧化活性的影响

Effects of spraying NaHS at different growth stages on osmotic adjustment substance and antioxidant activity in leaves of naked oat under salinealkali stress.

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