压砂地硒砂瓜自根苗/嫁接苗不同连作方式土壤微生物群落结构比较

doi:10.13292/j.1000-4890.202111.009

生态学杂志 ›› 2021, Vol. 40 ›› Issue (11): 3608-3619.doi: 10.13292/j.1000-4890.202111.009

压砂地硒砂瓜自根苗/嫁接苗不同连作方式土壤微生物群落结构比较

李靖宇^1,2*,张肖冲¹,田兴国¹,张琇^1,2

(¹北方民族大学生物科学与工程学院，银川 750021； ²宁夏特殊生境微生物资源开发与利用重点实验室，银川 750021)

出版日期:2021-11-10 发布日期:2022-05-10

Comparison of soil microbial community structure under different continuous cropping patterns of self-rooted and grafted seedlings of selenium-rich sand melon in artificial sand-covered soil.

LI Jing-yu^1,2*, ZHANG Xiao-chong¹, TIAN Xing-guo¹, ZHANG Xiu^1,2

(¹College of Biological Science & Engineering, North Minzu University, Yinchuan 750021, China; ²Ningxia Key Laboratory of Microbial Resources Development and Applications in Special Environment, Yinchuan 750021, China).

Online:2021-11-10 Published:2022-05-10

摘要/Abstract

摘要： 干旱半干旱区农业可持续发展不仅面临干旱胁迫，还面临连作障碍带来的负面影响。压砂方式为宁夏中部旱区硒砂瓜种植提供了有利条件。然而，长时间连作导致自根苗硒砂瓜大面积死亡，只能通过嫁接方式缓解连作障碍。对于不同连作方式（连作自根苗后直接连作嫁接苗以及连作自根苗后换砂处理后连作嫁接苗）对压砂地土壤微生物群落结构的影响缺乏深入认识。本文采用Illumina Miseq测序技术对不同连作方式压砂层土壤和土壤层细菌群落结构和真菌群落结构进行了分析。结果表明，连作方式和不同土层对细菌群落结构和真菌群落结构都有显著影响。连作方式S3（自根苗连作11年+换砂2年+嫁接苗连作3年）和S4（自根苗连作12年+嫁接苗连作4年）土壤层细菌群落结构发生显著变化的属主要包括Streptomyces、Gaiella、Ensifer、Nordella、Mesorhizobium；真菌群落结构发生显著变化的属主要包括Fusarium、Mortierella、Neocosmospora、Setophoma、Cladosporium、Chrysosporium、Periconia、Penicillium、Sarocladium等。而连作方式S4土壤层大量富集了Neocosmospora、Chrysosporium、Lectera，特别是Fusarium和paracylindrocarpon真菌类群，其中Fusarium的相对多度显著高于连作方式S3。这些结果为评价不同连作方式导致土壤微生物群落结构失衡提供科学数据。

关键词: 干旱区, 农业可持续发展, 连作障碍, 细菌群落结构, 真菌群落结构

Abstract: The sustainable development of agriculture in arid and semiarid areas faces both drought stress and the negative impacts of continuous cropping. In the arid area of central Ningxia, artificial sand-covered soil provides favorable conditions for planting selenium-rich sand melon. However, long-term continuous cropping has led to the death of selfrooted seedlings in large areas, which can only be alleviated by grafting. There is a critical need for in depth understanding of the effects of different continuous cropping patterns on the structure of soil microbial community. In this study, the bacterial and fungal community structure of the artificial sandy layer and soil layer under different continuous cropping patterns were analyzed by Illumina MiSeqsequencing technology. The results showed that continuous cropping patterns and different soil layers significantly affected bacterial and fungal community structures. The genera with significant changes in the bacterial community of soil layers in the cropping mode S3 (self-rooted seedlings with continuous cropping for 11 years + sand replacement for 2 years + grafted seedlings with continuous cropping for 3 years) and S4 (selfrooted seedlings with continuous cropping for 12 years + grafted seedlings with continuous cropping for 4 years) included Streptomyces, Gaiella, Ensifer,Nordella, and Mesorhizobium; the genera with significant changes in fungal community structure mainly included Fusarium, Mortierella,Neocosmospora, Setophoma, Cladosporium, Chrysosporium,Periconia, Penicillium, and Sarocladium. Neocosmospora, Chrysosporium, and Lectera were enriched in the soil layer of S4 under continuous cropping, especiallyFusarium and Paracylindrocarpon. The relative abundance of Fusarium in S4 was significantly higher than that in S3. These results provide scientific data related to the imbalance in soil microbial community structure caused by different continuous cropping patterns.

Key words: arid area, sustainable agricultural development, continuous cropping obstacle, bacterial community structure, fungal community structure.

李靖宇, 张肖冲, 田兴国, 张琇. 压砂地硒砂瓜自根苗/嫁接苗不同连作方式土壤微生物群落结构比较[J]. 生态学杂志, 2021, 40(11): 3608-3619.

LI Jing-yu, ZHANG Xiao-chong, TIAN Xing-guo, ZHANG Xiu. Comparison of soil microbial community structure under different continuous cropping patterns of self-rooted and grafted seedlings of selenium-rich sand melon in artificial sand-covered soil.[J]. Chinese Journal of Ecology, 2021, 40(11): 3608-3619.

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压砂地硒砂瓜自根苗/嫁接苗不同连作方式土壤微生物群落结构比较

Comparison of soil microbial community structure under different continuous cropping patterns of self-rooted and grafted seedlings of selenium-rich sand melon in artificial sand-covered soil.

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