丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响

doi:10.13292/j.1000-4890.202403.034

摘要/Abstract

摘要： 研究丛枝菌根真菌(AMF)接种与磷添加对干旱胁迫燕麦根系AMF侵染率、土壤微生物生物量和酶活性的影响，探讨其与燕麦产量的关系，为旱作区燕麦磷肥合理施用的菌根调控技术提供依据。试验采用盆栽控水，设置2个水分水平（正常供水，75%土壤相对含水量，W1；干旱胁迫，55%土壤相对含水量，W2）、3个施磷水平（0、20、40 mg·kg^-1，P0、P1、P2）、2个AMF水平（接种，AMF；不接种，NAMF），共12个处理。于燕麦拔节期、灌浆期、成熟期采集根系和土样，检测根系AMF侵染率，测定土壤MBC、MBN、MBP，土壤蔗糖酶、脲酶、碱性磷酸酶活性，及成熟期燕麦产量。结果表明：水分处理、磷处理和AMF处理均对各指标有显著影响，三因子在土壤MBN和土壤蔗糖酶活性上存在显著交互作用。干旱胁迫下，与未接种处理相比，接种AMF后，各指标均显著提高。P1下，燕麦生育期内AMF侵染率，土壤MBC、MBN、MBP，土壤蔗糖酶、脲酶、碱性磷酸酶活性及产量较P0显著提高的最大幅度分别达13.21%、52.26%、47.07%、88.94%、23.15%、15.44%、11.15%、17.16%，P2下各指标呈降低趋势。因此，接种AMF和适量增施磷肥是提高干旱胁迫燕麦土壤MBC、MBN、MBP，改善土壤蔗糖酶、脲酶、碱性磷酸酶活性，增加燕麦产量的有效途径，但产量尚达不到正常供水不接种水平。

关键词: 燕麦, 干旱胁迫, AMF, 磷, 土壤微生物生物量, 土壤酶活性

Abstract: The objectives were to investigate the effects of applying AMF and phosphorus on AMF infection of oat roots, soil microbial biomass, soil enzyme activity, as well as oat yield under drought stress, so as to develop AMF and phosphorus application guidelines for growing oat under drought. The experiment was conducted in pots for a total of 12 treatments, consisted of two levels of water supply, normal water supply (75% soil relative water content, W1) and drought stress (55% soil relative water content, W2), three levels of phosphorus supplement, 0, 20, and 40 mg·kg^-1 (P0, P1, P2), and two levels of AMF, inoculated (AMF) and not inoculated (NAMF). Root and soil samples were collected at the jointing, filling, and maturity stages to determine the AMF infection rate of oat roots, the soil MBC, MBN, and MBP, the soil sucrase, urease, and alkaline phosphatase activities. Oat yield was determined at maturity stage. The results showed that significant effects of soil water content, phosphorus, and AMF treatment were found on these indicators. Significant interactions among three factors were found on soil MBN and soil sucrose activities. Under drought stress, compared with non-inoculated AMF, inoculated AMF significantly increased these indicators. Compared to P0, the AMF infection rate, the soil MBC, MBN, and MBP, the soil sucrase, urease, and alkaline phosphatase activities and yield significantly increased by 13.21%, 52.26%, 47.07%, 88.94%, 23.15%, 15.44%, 11.15% and 17.16% (the maximum value) with P1 application in oat growth period, respectively. However, these indicators tended to be reduced with further increasing of phosphorus application (P2). Therefore, AMF inoculation and a moderate application of phosphorus were effective ways to increase the soil MBC, MBN and MBP, improve the activities of soil sucrase, urease and alkaline phosphatase, and increase oat yield under drought stress; however, the oat yield did not reach the level in normal water supply without AMF inoculation.

Key words: oat, drought stress, AMF, phosphorus, soil microbial biomass, soil enzyme activity

张斌, 吕玉峰, 李利, 冯美臣, 王超, 宋晓彦, 杨武德, 张美俊. 丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响[J]. 生态学杂志, 2024, 43(3): 644-655.

ZHANG Bin, LYU Yufeng, LI Li, FENG Meichen, WANG Chao, SONG Xiaoyan, YANG Wude, ZHANG Meijun. Effects of AMF and phosphorus application on microbial biomass and enzyme activities in oat soil under drought stress.[J]. Chinese Journal of Ecology, 2024, 43(3): 644-655.

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丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响

Effects of AMF and phosphorus application on microbial biomass and enzyme activities in oat soil under drought stress.

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