• 研究报告 •

一株低温纤维素降解菌的筛选与产酶条件优化

1. 1东北农业大学资源与环境学院， 哈尔滨 150030； 2东北农业大学工程学院， 哈尔滨 150030；3元山农业综合大学国土环境保护系， 元山 950003, 朝鲜
• 出版日期:2021-04-10 发布日期:2021-04-13

Screening of a low-temperature cellulose-degrading bacterium and optimization of cellulase production conditions.

SHAN Jian-rong1, QUAN Xin1, ZHU Yong-zhe3, XING Yu1, ZHANG Xu1, WANG Hong-yan1*, FAN Jin-xia2*

1. (1College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China; 2College of Engineering, Northeast Agricultural University, Harbin 150030, China; 3Department of Land and Environmental Protection,Wonsan University of Agriculture,Wonsan,Pyongyang 950003, Democratic People’s Republic of Korea).
• Online:2021-04-10 Published:2021-04-13

Abstract: An effective solution to resolve the problems of slow start of cow manure composting and long fermentation cycle in winter is the addition of lowtemperature cellulose-degrading microbe to the pile. However, few types of low-temperature cellulose-degrading microbe are available. In this study, one low-temperature and high-efficiency cellulose-degrading strain was isolated from frozen cow dung under low temperature conditions, which was named YSX-3. It was identified as Pseudomonas. In order to obtain its optimal enzyme production condition, on the basis of single factor optimization, incubation time, incubation temperature, initial pH value and inoculum amount are considered as independent variables, and cellulase activity as the response variable. Response surface analysis (RSM) was used to analyze the relationship between response variable and the independent variables. The results showed that the optimal enzyme production condition of YSX-3 strain was as follows: inoculating amount 4%, culture temperature 9 ℃, culture time 71 h and initial pH 6.5. Under this condition, the cellulase activity was 130.21 U·mL-1, which was 1.3 times higher than that before optimization. The optimized YSX-3 strain was inoculated into the manure pile. On the fifth day, the high-temperature period came (>50 ℃), the temperature reached up to 62.5 ℃, and the high temperature period lasted for nine days. At the end of composting, the degradation rate of cellulose reached 45.2%, while that in the control group was only 15.4%.