基于高通量测序技术分析患病与健康虾夷扇贝(Patinopecten yessoensis)闭壳肌菌群多样性

生态学杂志

基于高通量测序技术分析患病与健康虾夷扇贝(Patinopecten yessoensis)闭壳肌菌群多样性

窦妍¹,赵晓伟¹,丁君^1*,刘圣聪²,张涛²

(¹大连海洋大学农业部北方海水增养殖重点实验室，辽宁大连 116023； ²大连天正实业有限公司，辽宁大连 116023)

出版日期:2016-04-10 发布日期:2016-04-10

Application of high-throughput sequencing for analyzing bacterial diversity in the adductor muscle of sick and healthy Patinopecten yessoensis.

DOU Yan¹, ZHAO Xiao-wei¹, DING Jun^1*, LIU Sheng-cong², ZHANG Tao²

(Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, Liaoning, China; Dalian Tianzheng Industry Co., Ltd., Dalian 116023, Liaoning, China).

Online:2016-04-10 Published:2016-04-10

摘要/Abstract

摘要： 本研究取自同一养殖海域不同浮筏养殖笼中健康和患脓包病虾夷扇贝（Patinopecten yessoensis）样品，健康虾夷扇贝样品标记为CK，患病样品分别标记为S1、S2和S3，使用MiSeq高通量测序技术比较不同生存状态的虾夷扇贝的微生物组成与差异。结果表明：4个样品中菌群多样性表现为S3>CK>S1>S2；4个样品中的细菌可归为24个门，主要门类有变形菌门、拟杆菌门、浮霉菌门、梭杆菌门、厚壁菌门、酸杆菌门、放线菌门、硝化螺旋菌门、绿菌门和绿弯菌门；在健康虾夷扇贝样品中，变形菌门占绝对优势，占整个菌群的95.9%，患病虾夷扇贝样品中，S1和S3中优势类群均为变形菌门，分别占整个菌群的80.4%和86.8%，S2中优势菌群为拟杆菌门，占56.9%。

关键词: 节能减碳效率, 非期望产出DEA模型, CO₂, 辽宁省

Abstract: Healthy and sick samples of Patinopecten yessoensis were taken from different culturing cages. The healthy samples were tagged as CK and the sickness samples were tagged as S1, S2 and S3 respectively. Using MiSeq sequencing method, we compared the microbial structure and variation of P. yessoensis in different healthy status. The results showed that the bacterial diversity was highest in the S3, followed by the CK, S1 and S2. In total, 24 phyla were identified in this study. Among these phyla, Proteobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, Firmicutes, Acidobacteria, Actinobacteria, Nitrospirae, Chlorobi and Fusobacteria took up a large proportion. Proteobacteria were absolutely dominant in the adductor muscle of healthy P. yessoensis, accounting for 95.9% in the entire bacterial community. In the adductor muscle of the sick P. yessoensis, the dominant groups of S1 and S3 were Proteobacteria, accounting for 80.4% and 86.8% respectively, while Bacteroidetes (56.9%) were the dominant phyla in S2.

Key words: Liaoning Province, CO₂, energy saving and carbon emission reduction efficiency, undesirable output DEA model

窦妍1,赵晓伟1,丁君1*,刘圣聪2,张涛2. 基于高通量测序技术分析患病与健康虾夷扇贝(Patinopecten yessoensis)闭壳肌菌群多样性[J]. 生态学杂志.

DOU Yan1, ZHAO Xiao-wei1, DING Jun1*, LIU Sheng-cong2, ZHANG Tao2. Application of high-throughput sequencing for analyzing bacterial diversity in the adductor muscle of sick and healthy Patinopecten yessoensis.[J]. cje.

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