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菊芋蔗糖代谢相关产物与关键酶基因对高温的响应

闻奋亮,隆小华,岳杨,何腾飞,高秀美,周兆胜*   

  1. (南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095)
  • 出版日期:2020-01-10 发布日期:2020-01-10

Responses of related products and key enzyme genes in sucrose metabolic pathway to high temperature in Jerusalem artichoke.

WEN Fen-liang, LONG Xiao-hua, YUE Yang, HE Teng-fei, GAO Xiu-mei, ZHOU Zhao-sheng*   

  1. (College of Resources and Environmental Sciences/Jiangsu Province Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China).
  • Online:2020-01-10 Published:2020-01-10

摘要: 蔗糖是一类重要的碳水化合物,其代谢与植物生长发育及抵抗胁迫等有密切的关系。蔗糖合成酶(SUS)、蔗糖磷酸合成酶(SPS)与蔗糖转化酶(INV)是参与蔗糖代谢的三类关键酶。本研究依据转录组测序数据,从能源植物菊芋中鉴定了2个SUS、2个SPS和7个INV基因(GenBank No: MK38694353)。生物信息学分析表明,菊芋SUS、SPS和INV的氨基酸序列与其他物种具有较高的相似性,均属于亲水性蛋白。在25、30 °C处理10、15、20 d的菊芋幼苗叶片中,这三种基因家族成员呈现不同的表达模式;除可溶性总糖含量减少外,果糖、蔗糖、蔗果三糖等含量没有发生明显变化。表明高温下幼苗蔗糖代谢关键酶基因发生了响应,蔗糖代谢处于平衡状态,显示了菊芋对高温的良好耐受性。

关键词: 时空格局, 产业结构, 吉林省, 生态化

Abstract: Sucrose is an important type of carbohydrates, the metabolism of which is closely related to plant growth, development and stress adaptation. Sucrose synthases (SUS), sucrose phosphate synthases (SPS) and sucrose invertases (INV) are three kinds of key enzymes involved in sucrose metabolism. Based on the transcriptome data, we identified 2 SUS, 2SPS and 7 INV genes (GenBank No: MK38694353) from Jerusalem artichoke, a bioenergy plant species. Bioinformatics analysis showed that SUS, SPS, and INV belonged to hydrophilic proteins, whose amino acid sequences had high similarity with other species. The three kinds of genes exhibited different expression patterns in the leaves of Jerusalem artichoke seedling treated at 25 ℃ and 30 ℃ for 10, 15, and 20 days, respectively. However, the product contents associated with sucrose metabolism, such as fructose, sucrose, and 1-kestose, had no significant changes, except for the total soluble sugar, the content of which was significantly reduced. This result indicated that these key enzyme genes responded to high temperature, and that sucrose metabolism was in a balanced state, reflecting that Jerusalem artichoke had a good tolerance ability to high temperature.

Key words: ecologicalization, industrial structure, spatio-temporal pattern, Jilin Province.