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东北红豆杉幼苗黄酮类化合物含量变化及其对气候因子的响应

张诗行,刘艳红*   

  1. (北京林业大学森林资源与生态系统过程北京市重点实验室, 北京 100083)
  • 出版日期:2020-01-10 发布日期:2020-01-10

Variation of flavonoid content in Taxus cuspidata seedlings and its responses to climate factors.

ZHANG Shi-hang, LIU Yan-hong*   

  1. (Beijing Key Laboratory of Forest Resources and Ecosystem Process, Beijing Forestry University, Beijing 100083, China).
  • Online:2020-01-10 Published:2020-01-10

摘要: 植物在进化适应过程中形成了一套对环境变化响应的防御体系,其中黄酮类化合物作为植物体内重要的次生代谢产物,是表征植物化学防御能力的重要指标。本研究将5个种源东北红豆杉幼苗分别移栽到4个试验地(37°N—43°N),利用多酚测量仪Multiplex3(Force-A,France)对其叶片表皮黄酮类化合物含量进行无损测定,探讨随试验地、种源地气候因子的变化,东北红豆杉幼苗黄酮类化合物含量的变化规律。结果表明:试验地环境变化对类黄酮指数、花青素指数变化的相对贡献率大于种源地、试验地与种源地交互效应的相对贡献率;试验地气候因子对类黄酮指数、花青素指数解释率(49.8%、55.4%)分别高于种源地气候因子的解释率(35.3%、29.2%);东北红豆杉幼苗类黄酮指数、花青素指数随试验地纬度的升高而下降;试验地3类气候因子方差分解结果显示,光照因子对类黄酮指数、花青素指数变化的单独解释率最高;种源地气候因子方差分解结果显示,水分因子对类黄酮指数、花青素指数变化的单独解释率最高;试验地环境变化对东北红豆杉幼苗黄酮类化合物含量变化起主要作用;东北红豆杉幼苗在低纬度试验地表现出更强的化学防御能力;光照因子在短期影响东北红豆杉幼苗黄酮类化合物含量变化中起主导作用,而长期影响黄酮类化合物含量变化则转变为水分因子起主导作用。

Abstract: Plants form a defense system that responds to environmental change during evolutionary adaptation. Flavonoids, as vital secondary metabolites in plants, are important indicators of plant chemical defense capability in the defense system. In this study, seedlings ofTaxus cuspidatafrom five provenances were transplanted to four sites (37°N—43°N). The flavonoid content in leaf epidermis of T. cuspidata seedlings was determined by Multiplex-3 (Force-A, France) non-destructively. We analyzed the change patterns of flavonoid content in the leaf epidermis of T. cuspidata seedlings which was influenced by climate factors in the test sites and provenances. The relative contribution rate of environmental change in the test sites to the changes of flavonol index (FLAV) and anthocyanin index (ANTH) of T. cuspidata seedlings were higher than that of environmental change in the provenances and the interaction effect between the test sites and the provenances. The explanatory rates of climate factors in the test sites on FLAV and ANTH (49.8%, 55.4%) were higher than those of climatic factors in the provenances (35.3%, 29.2%). The FLAV and ANTH of T. cuspidataseedlings decreased with the increase of latitude in the test sites. The results of variation partitioning of climate factors in the test sites indicated that illumination had the highest explanation rate for the changes of FLAV and ANTH. The results of variation partitioning of climatic factors in the provenances revealed that waterrelated factors had the highest explanation rate for the change of FLAV and ANTH. The environmental change in the test sites played a leading role in driving the change of flavonoid content in T. cuspidataseedlings.T. cuspidata seedlings had stronger chemical defense ability in low latitude test sites. Illumination played a dominant role in driving the changes of flavonoid content in T. cuspidata seedlings in the short term, whereas water played a dominant role in the long term.