内生真菌印度梨形孢对旱稻苗期生长及抗旱性的影响

生态学杂志

内生真菌印度梨形孢对旱稻苗期生长及抗旱性的影响

韦巧,武美燕,张文英^*,徐乐,陈建芳,潘锐,田小海

（主要粮食作物产业化湖北省协同创新中心，湿地生态与农业利用教育部工程研究中心，长江大学，湖北荆州 434025）

出版日期:2018-09-10 发布日期:2018-09-10

Effect of the endophytic fungus Piriformospora indicaon the growth and drought tolerance of rice seedling under drought stress.

WEI Qiao, WU Mei-yan, ZHANG Wen-ying^*, XU Le, CHEN Jian-fang, PAN Rui, TIAN Xiao-hai

(Hubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, Hubei, China).

Online:2018-09-10 Published:2018-09-10

摘要/Abstract

摘要： 通过平板共生培养及20%聚乙二醇（polyethylene glycol，PEG）模拟干旱胁迫，研究了内生真菌印度梨形孢（Piriformospora indica）对旱稻生长及其抗旱性的影响。结果表明：印度梨形孢与旱稻品种“绿旱1号”共生培养23 d后，植株生物量显著增加，总根长和总根表面积增加1倍，根尖数增加3倍；20% PEG模拟干旱胁迫下，接种印度梨形孢幼苗叶片发生萎蔫卷曲明显延缓，旱稻叶片温度升高，且随着干旱处理时间的延长，接种印度梨形孢处理比不接种处理的叶温差大；抗旱相关基因OsMYB2、OsCIPK3、OsCDPK7、OsCATB在旱稻叶片中的表达量随干旱处理时间的延长呈不同程度上调；印度梨形孢能够促进旱稻幼苗生长、提高其抗旱性。

关键词: 增雨, 降水格局, 土壤酸化, 氮沉降

Abstract: By Petri dish culture, an experiment of simulated drought stress was carried out by 20% polyethylene glycol (PEG 6000), to examine the effects of endophytic fungus (Piriformospora indica) on the growth and drought resistance of upland rice. The results showed that the biomass of rice seedlings significantly increased after colonization with P. indica for 23 days, with two-folds in total root length and total root surface area, and four-folds in the number of root tips. Under 20% PEG drought stress, the progress of leaf wilting and curling was slowed down, and the leaf temperature raised in the treatment with P. indicainoculation. With the extension of drought stress duration, the difference of leaf temperature in the treatment with P. indica inoculation was greater than that in the non-inoculated treatment. The expression levels of responsive genes related to drought stress, including OsMYB2, OsCIPK3,OsCDPK7 and OsCATB, were up-regulated in leaves of upland rice induced by P. indica with the extension of drought stress duration. In conclusion, P. indica inoculation could promote the growth and enhance the drought tolerance of upland rice.

Key words: N deposition, precipitation regime, soil acidification, water addition

韦巧,武美燕,张文英,徐乐,陈建芳,潘锐,田小海. 内生真菌印度梨形孢对旱稻苗期生长及抗旱性的影响[J]. 生态学杂志.

WEI Qiao, WU Mei-yan, ZHANG Wen-ying, XU Le, CHEN Jian-fang, PAN Rui, TIAN Xiao-hai. Effect of the endophytic fungus Piriformospora indicaon the growth and drought tolerance of rice seedling under drought stress.[J]. cje.

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