李坤1,朱绍坤1,郭修武1*,谢洪刚2,郭印山1,刘镇东1
LI Kun1, ZHU Shao-kun1, GUO Xiu-wu1*, XIE Hong-gang2, GUO Yin-shan1, LIU Zhen-dong1
摘要: In order to explore the correlation between soil microbial community function and plant growth, using 30year continuous cropping soil of grapevines as research object, we studied the effects of sterilization of continuous cropping soil at different temperatures on the growth of grapevines and the microbial community function of rhizosphere soil. The results showed that plant height and stem diameter of grapevines grown in continuous cropping soil were lower than those in the other treatments. With the increasing sterilization temperature, the plant height and stem diameter of grapevines increased. The ratio of bacteria to fungi in rhizosphere soil increased with the increasing sterilization temperature. The activity of rhizosphere microbes using carbon source was in order of continuous cropping soil sterilized at 100 ℃ > noncontinuous cropping soil > continuous cropping soil sterilized at 60 ℃ > continuous cropping soil. The regression analysis showed that amino acid (carbon source) in Biolog ECOplate had a significant correlation with microbial metabolic activity of rhizosphere soil. PCA analysis showed that lysine in root exudate had a highest contribution to the variance of principal components in each treatment, and it may play an important role in the obstacle of continuous cropping of grapevines.