Habitat use of seven Burmese pythons was analyzed using radio track, GPS position, quadrat investigation and resource selection functions in Hainan Yinggeling National Nature Reserve from March to June in 2014. A total of 80 utilized quadrats and 75 random quadrats were measured. The results showed that the habitats that the Burmese pythons occupied during early release stages tended to be shrub and grassland. The habitats were characterized as lower elevation, sunny slope, lower forest canopy density, environmental temperature (27.19±2.44) ℃, higher illumination intensity, far from the disturbance and close to the water. Resource selection function on habitat use of Burmese python was expressed as: logit(p)=-0.21 - 2.77 × environmental temperature -2.20 × slope + 1.44 × illumination intensity + 1.21 × vegetation type -1.19 × forest canopy density. In this model, habitat use of Burmese python was negatively correlated with environmental temperature, slope and forest canopy density, and positively related to illumination intensity and vegetation type. According to the fitted resource selection function, habitat use probability of Burmese python was expressed as P=elogit(P)/\[1+elogit(P)\], and the accuracy rate of this model was 90.7% (R²=0.843). The main factors affecting habitat use of the Burmese pythons during early reintroduction included environmental temperature, illumination intensity and vegetation type.