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水体N/P对塔玛亚历山大藻响应紫外辐射的影响

李磊1,关万春1,陈少波1,2,谢起浪1*   

  1. (1温州医科大学生命科学学院海洋生物技术系, 浙江温州 325035; 2浙江省海洋水产养殖研究所, 浙江温州 325005)
  • 出版日期:2016-02-10 发布日期:2016-02-10

Effect of water N/P ratio on the response of Alexandrium tamarense to UV radiation.

LI Lei1, GUAN WAN-chun1, CHEN Shao-bo1,2, XIE Qi-lang1*   

  1. (1Department of Marine Biotechnology, School of Life Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China; 2Zhejiang Mariculture Research Institute, Wenzhou 325005, Zhejiang, China)
  • Online:2016-02-10 Published:2016-02-10

摘要: 本文探讨了塔玛亚历山大藻(Alexandrium tamarense)在5种N/P(1∶1、16∶1、50∶1、100∶1和200∶1)条件下适应培养14 d后藻细胞生长和色素的差异。然后将适应培养14 d的藻细胞分为3种辐射处理\[可见光(P)、可见光+紫外辐射A(PA)和可见光+紫外辐射A+B(PAB)\],探讨藻细胞对紫外辐射响应机制的差异。采用人工紫外灯作为光源,检测3种辐射处理60 min有效光化学效率的变化和快速荧光曲线。结果表明:当N/P为16∶1时,塔玛亚历山大藻的生长最快,叶绿素a(Chl-a)和类胡萝卜素(Caro)的含量可达3.06×10-5和2.10×10-5 μg·cell-1;提高或降低N/P都会抑制生长,生长速率与N/P之间符合一元二次方程(R2>0.98),而色素含量与N/P之间无此规律;经不同N/P培养14 d后,藻细胞接受3种辐射处理60 min,其光化学效率的变化符合一元指数方程(R2>0.97);与P处理相比,PA处理和PAB处理的光化学效率都显著下降,且光化学效率的降低程度与藻细胞光系统II D-1-蛋白的修复与损伤速率比值(r/k)存在显著的负线性关系(R2>0.98);紫外辐射对塔玛亚历山大藻的抑制率受到水体中N/P的影响,其规律符合一元二次方程(R2>0.95);N/P=16∶1时,r/k最大,光化学效率下降程度和紫外辐射抑制率都最小;因此,N/P=16∶1是藻细胞耐受紫外线辐射的最佳营养盐比例,N/P可通过改变塔玛亚历山大藻细胞D1蛋白的r/k来影响藻细胞对紫外线辐射的响应。

关键词: 茶树, 土壤微生物群落, 宿根连作, 磷脂脂肪酸, 微生物碳源利用率

Abstract: Alexandrium tamarense, a typical species around China coastal water zone, was cultured under conditions of five N/P ratios (1∶1, 16∶1, 50∶1, 100∶1, 200∶1) for 14 days. The growth rates and pigment contents were measured at the end of the experiment. Then the cultures were exposed under three types of radiation (visible light, P; visible light + ultraviolet A, PA; visible light + ultraviolet A+B, PAB). The effective photochemical efficiency and exposure response curves (ERC) were detected after 60min exposure. The results showed the fastest growth rate and highest pigment contents (Chl-a: 3.06×10-5 μg·cell-1 and Caro: 2.10×10-5 μg·cell-1) were observed in the 16∶1 (N/P) treatment. However, the growth rates and pigment contents declined significantly when the N/P ratio in the media increased or decreased. The growth rate was a function of N/P ratio according to a twoorder equation (R2>0.98). The variation of effective photochemical efficiency was fitted well by a firstorder exponential equation (R2>0.97). The effective photochemical efficiency decreased under PA and PAB treatments compared to that under P treatment. There was a negative linear relationship between the decreased degree of the effective photochemical efficiency and the ratio of algal cell photosynthetic system II D1 protein repair rate and damage rate (r/k) (R2>0.98). The dependency of UVinduced photoinhibition in A. tamarense on the N/P ratio was fitted well by a two-order equation (R2>0.95). The r/k ratio was the highest and the decreased degree of the effective photochemical efficiency and the photoinhibition rate were the lowest in the 16∶1 (N/P) treatment. Therefore, the N/P ratio of 16∶1 is the optimum condition for the algal cells to defense the UVinduced photoinhibition by the alteration of r/k ratio.

Key words: tea plant, soil microbial community, phospholipid fatty acid., microbial utilization rate of carbon source, ratooning continuous cropping