泥鳅对饲料中添加转融合基因Cry1Ab/Ac水稻花粉的响应

生态学杂志

泥鳅对饲料中添加转融合基因Cry1Ab/Ac水稻花粉的响应

李菲¹,刘雨芳^1**,孙远东¹,莫书银¹,肖璐¹,谭树华¹,刘文海¹,桂芳艳¹,戈峰^2

(¹ 湖南科技大学生命科学学院，园艺作物病虫害治理湖南省重点实验室，湖南湘潭 411201； ^2中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室，北京 100101)

出版日期:2014-10-10 发布日期:2014-10-10

Response of Misgurnus anguillicaudatus to feed with transgenic Cry1Ab/Ac rice pollen added.

LI Fei¹, LIU Yu-fang^1**, SUN Yuan-dong¹, MO Shu-yin¹, XIAO Lu¹, TAN Shu-hua¹, LIU Wen-hai¹, GUI Fang-yan¹, GE Feng^2

(¹ College of Life Science, Hunan University of Science and Technology, Hunan Province Key Laboratory for Integrated Management of the Pests and Diseases on Horticultural Crops, Xiangtan 411201, Hunan, China; ² Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)
.

Online:2014-10-10 Published:2014-10-10

摘要/Abstract

摘要：

以转Cry1Ab/Ac抗虫水稻华恢1号(HH1)花粉为添加剂，配制4种不同花粉含量(1%、5%、10%、20%）的饲料，以非转基因亲本水稻明恢63(MH63)花粉为对照，常规泥鳅饲料（不添加花粉）为阴性对照，投喂100 d，通过泥鳅的生长指标和肝脏生理酶活性评价转Cry1Ab/Ac抗虫水稻HH1号花粉对泥鳅的安全性。结果表明，在饲料中分别添加相同比例的HH1与MH63花粉，两组泥鳅的增重率、脏体比和肝体比指数均无显著差异，同时对泥鳅的肝脏抗氧化保护酶SOD、CAT活性与解毒酶GST活性也均无显著影响。饲料中花粉含量为20%时，在饲养100 d后，转Cry1Ab/Ac水稻花粉对泥鳅的生长性能与肝脏生理酶代谢功能没有明显影响。因此，转Cry1Ab/Ac抗虫水稻HH1号花粉对泥鳅无明显的生态风险。

关键词: 饱和脉冲, 多相脉冲, 光下最大荧光, 光合参数

Abstract: As an additive, the pollen of transgenic Cry1Ab/Ac insectresistant rice (HH1) was added to the normal feed of Misgurnus anguillicaudatus, which made the artificial feed containing 1%, 5%, 10% and 20% pollen, respectively. M. anguillicaudatus were cultured in laboratory for 100 days fed on one of the artificial feeds mentioned above. Feed with the pollen of nonCry1Ab/Ac parent rice (MH63) added was used as a control, while the regular feed without any pollen was used as a negative control. The ecological safety of transgenic Cry1Ab/Ac rice pollen on M. anguillicaudatus was evaluated by testing the growth performance and activities of physiological enzymes in liver of M. anguillicaudatus. The results showed that no significant differences were found in the weight gain rate, viscera somatic index and liver somatic index of M. anguillicaudatus feeding transgenic Cry1Ab/Ac rice pollen compared to that in the control groups. The activities of two antioxidant protective enzyme, SOD and CAT, and detoxification enzyme GST of M. anguillicaudatus had no significant difference either. With up to 20% pollen content of transgenic Cry1Ab/Ac rice in feed, no obvious influences on the growth performance and physiological metabolism of liver function of M. anguillicaudatus were found after feeding 100 days. The results suggested that the pollen of transgenic Cry1Ab/Ac rice would have no obvious ecological risk on M. anguillicaudatus.

Key words: rectangular flush, maximum chlorophyll fluorescence under illumination, photosynthetic parameters, multiphase flush

李菲1,刘雨芳1**,孙远东1,莫书银1,肖璐1,谭树华1,刘文海1,桂芳艳1,戈峰2. 泥鳅对饲料中添加转融合基因Cry1Ab/Ac水稻花粉的响应[J]. 生态学杂志.

LI Fei1, LIU Yu-fang1**, SUN Yuan-dong1, MO Shu-yin1, XIAO Lu1, TAN Shu-hua1, LIU Wen-hai1, GUI Fang-yan1, GE Feng2. Response of Misgurnus anguillicaudatus to feed with transgenic Cry1Ab/Ac rice pollen added.[J]. cje.

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