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镉胁迫对烟草愈伤组织抗氧化系统的影响

高阳,娄虹,李淑媛,王红艳,阮亚男**   

  1. (辽宁大学生命科学院, 沈阳 110036)
  • 出版日期:2014-05-10 发布日期:2014-05-10

Effect of cadmium stress on antioxidative system in tobacco callus.

GAO Yang, LOU Hong, LI Shu-yuan, WANG Hong-yan, RUAN Ya-nan**   

  1. (College of Life Sciences, Liaoning University, Shenyang 110036, China)
  • Online:2014-05-10 Published:2014-05-10

摘要: 镉(Cd)是烟草(Nicotiana tabacum L.)生长的非必需元素,但容易在烟草体内残留,危害其生长发育。本实验设定0~50 mg·L-1 7个梯度浓度的Cd2+,以MS+1.0 mg·L-1 6BA+0.5 mg·L-1 NAA为基本培养基,诱导产生烟草叶片愈伤组织。待45 d愈伤组织丰富之后,每间隔3 d测定一次烟草愈伤组织内抗氧化系统相关酶活性、活性氧水平及丙二醛含量。结果表明:短期(45~51 d)Cd2+胁迫可导致烟草愈伤组织内活性氧水平升高,抗氧化酶系统活性增强,但长期(51~57 d)高浓度Cd2+胁迫导致抗氧化系统活性下降,膜质过氧化程度加重。

关键词: 二化螟, 温度, 触角电位, 性信息素通讯

Abstract: Cadmium is a non-essential element for plant growth, however, it could be absorbed and remained easily, and make an obvious damage to tobacco (Nicotiana tabacum L.). In this study, tobacco leaves were induced to produce callus by the minimal medium (MS + 1.0 mg·L-1 6BA + 0.5 mg·L-1 NAA). After 45 days, with a large amount of callus, the activities of related enzymes (SOD, CAT, APX, MDAR, GR and DHAR) in the antioxidant system, the levels of reactive oxygen species (O-·2, H2O2), and malondialdehyde (MDA) content were measured every three days. The results showed that the reactive oxygen species enhanced, and the activities of antioxidantive enzymes increased in tobacco callus under the cadmium stress within 45-51 days. However, after 51-57 days the activities of antioxidantive system, and membrane lipid peroxidation were aggravated, indicating that the increasing activities of antioxidantive system could not resist the longterm (54-57 days) high cadmium stress.

Key words: electroantennograph, Chilo suppressalis, temperature, sex pheromone communication