磷脂脂肪酸分析方法在微生物生态学中的应用

摘要/Abstract

摘要： 磷脂脂肪酸分析方法(PLFA)是基于生物化学手段的一种微生物生态学研究新技术,它具有对细胞生理活性没有特殊的要求,对样品保存时间也要求不高等优点,由样品中所有微生物提供信息,是一种快捷、可靠的分析方法.本文介绍了PLFA在微生物生态学研究中的应用,主要包括对微生物群落生物量、群落结构和功能及其变化,指示特定微生物以及营养状况方面的研究.

关键词: 黑土, 有机无机复合体, 腐殖质组成, 胡敏酸类型

Abstract: Phospholipid fatty acid (PLFA) analysis,based on modern biochemical techniques,is widely used in the research of microbial ecology nowadays.It shows many advantages over the conventional culturing methods such as accurate,quantitative,simple in operation,as well as no strict requirements for the microbial physiological status and sample conservation duration.The information provided by PLFA can present all the viable members of the microbial community.This paper reviewed the principles and applications of PLFA in the research of microbial ecology,including the biomass in the microbial community,the community structure and function and their changes,the indication of certain microbial species or genus,and the nutritional status.

Key words: Black soil, Organo-mineral complex, Composition of humus, Types of humic acid

中图分类号:

X172

陈振翔, 于鑫, 夏明芳, 戴朝霞, 孙成. 磷脂脂肪酸分析方法在微生物生态学中的应用[J]. 生态学杂志, 2005, (7): 828-832.

CHEN Zhenxiang, YU Xin, XIA Mingfang, DAI Zhaoxia, SUN Cheng . Application of phospholipid fatty acid (PLFA) analysis in microbial ecology[J]. cje, 2005, (7): 828-832.

参考文献

[1] 于鑫,张晓健,王占生.2003.饮用水生物处理的膜生态学研究进展[J].中国给水排水,19(6):29～31.
[2] 齐鸿雁,薛凯,张洪勋.2003.磷脂脂肪酸谱图分析方法及其在微生物生态学领域的应用[J].生态学报,23(8):1576～1582.
[3] 陈进才,林庆梅,郑天凌,等.1999.利用可提取性磷脂表征沉积物中微生物的生物量[J].台湾海峡,18(4):407～412.
[4] 林庆梅,陈进才,李玉桂,等.2001.海洋沉积物中磷脂类化合物中脂肪酸的GC/MS分析[J].分析仪器,2:19～21.
[5] 姚槐应,何振立,黄昌勇.2003.不同土地利用方式对红壤微生物多样性的影响[J]水土保持学报,17(2):51～54.
[6] Albrechtsen HJ, Heron G, Christensen TH. 1995. Limiting factors for microbial Fe( Ⅲ )-reduction in a landfill leachate polluted aquifer (Vejen, Denmark) [J]. FEMS Microbiol. Ecol., 16: 233～ 247.
[7] Bardgett RD, Kandeler E, Tscherko D, et al. 1999. Below-ground microbial community development in a high temperature world[J]. oikos,85:193～ 203.
[8] Bomeman J, Skroch PW, Palus JA, et al. 1996. Molecular microbial diversity of an agricultural soil in Wisconsin[J]. Appl. Environ. Microbiol . , 62(6): 1935～1943.
[9] Bowman JP, McCammon SA, Gibson JAE, et al. 2003. Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments[J]. Appl. Environ. Microbiol., 69: 2448～ 2462.
[10] Bowman JP, Jimenez L, Rosario I, et al. 1993. Characterization of the methanotrophic bacterial community present in a trichloroethylene-contaminated subsurface groundwater site [J].Appl . Environ. Microbiol . , 59:2380～2387.
[11] Cha DK, et al. 1999. Fatty acid methyl ester (FAME) analysis for monitoring Nocardia levels in activated sludge [J]. Wat.Res., 38(8): 1964 ～ 1966.
[12] Christopher T, Green K, Scow M. 2000. Analysis of phospholipid fatty acids (PLFA) to characterize microbial communities in aquifers[J]. Hydrogeology J., 8:126～141.
[13] Fang J, Barcelona MJ. 1998. Structural determination and quantitative analysis of bacterial phospholipids using liquid chromatography/ electrospray ionization/mass spectrometry [J]. J . Microbiol. Methods, 33: 23 ～ 35.
[14] Fredrickson JK, McKinley JP, Bjomstad BN, et al. 1997. Poresize constraints on the activity and survival of subsurface bacteria in a late Cretaceous shale-sand stone sequence, northwestern New Mexico[J]. G-eomicrobiology, 14:183 ～ 202.
[15] Fritze H, Pietikainen J, Pennanen T. 2000. Distribution of microbial biomass and phospholipid fatty acids in podzol profiles under coniferous forest[J]. Euro. J. Soil Sci., 51: 565 ～ 573.
[16] Frostegard A, Baath E. 1996. The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil[J]. Biol.Fert. Soils, 22: 59 ～ 65.
[17] Frostegard A, Tunlid A, Baath E. 1991. Microbial biomass measured as total lipid phosphate in soils of different organic content[J].J. Microbiol. Method, 14:151 ～ 163.
[18] Frostegard A, Tunlid A, Baath E. 1993. Phospholipid fatty acid composition, biomass and activity of microbial communities from two soil types experimentally exposed to different heavy metals[J]. Appl. Environ. Microbiol., 59: 3605 ～ 3617.
[19] Haack SK, Garchow H, Odelson DA, et al. 1994. Accuracy, reproducibility, and interpretation of fatty acid methyl ester profiles of model bacterial communities[J]. Appl. Environ. Microbiol.,60: 2483 ～ 2493.
[20] Jain DK, Stroes-Gascoyne S, Providenti M, et al. 1997. Characterization of microbial communities in deep groundwater from granitic rock[J]. Can J. Microbiol., 43: 272～ 283.
[21] Kandeler E, Tscherko D, Bruce KD, et al. 2000. Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soil[J]. Biol. Fert. Soils, 32: 390～ 400.
[22] Kieft TL, Ringelberg DB, White DL. 1994. Changes in esterlinked phospholipid fatty acid profiles of subsurface bacteria during starvation and desiccation in a porous medium[J]. Appl. Environ. Microbiol., 60: 3292～ 3299.
[23] Lechevalier MP. 1997. Lipids in bacterial taxonomy A taxonomist's view[J]. Crit Rev. Microbiol. , 7:109～210.
[24] Liu WT, Marsh TL, Cheng H, et al. 1997. Characterization of Microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA[J]. Appl.Environ. Microbiol., 63(11) :4516～ 4522.
[25] Ludvigsen L, Albrechtsen HJ, Ringelberg DB, et al. 1999. Distribution and composition of microbial populations in a landfill leachate contaminated aquifer (Grindsted, Denmark) [J]. Microb . Ecol., 37:197～ 207.
[26] Madigan MT, Martinko JM, Parkerer J. 1999. Brock-Biology of microorganisms. 9th edition [M]. London: Prentice Hall, 53 ～55.
[27] Madsen EL, Sinclair JL, Ghiorse WC. 1991. In situ biodegradation microbiological patterns in a contaminated aquifer[J]. Science, 252: 830～ 833.
[28] Makula RA. 1978. Phospholipid composition of methaneutilizing bacteria[J]. J. Bacteriol, 134: 771～ 777.
[29] Pennanen T, Frostegard A, Fritze H, et al. 1996. Phospholipid acid composition and heavy metal tolerance of soil microbial communities along two heavy metal-polluted gragients in coniferous forests [J]. Appl. Environ. Microbiol., 62(2) :420～428.
[30] Perry GJ, Volkman JK, Johns RB, et al. 1979. Fatty acids of bacterial origin contemporary sedimens [J]. Geochim Cosmochim. Acta, 43: 1715～ 1725.
[31] Petersen S, Klug M. 1994. Effects of sieving, storage, and incubation temperature on the phospholipid fatty acid profile of a soil microbial community[J]. Appl. Environ. Microbiol., 60:2421～ 2430.
[32] Priha O, Susan J, et al. 2001. Microbial community structure and characteristics of the organic matter in soils under Pinus sylvestris, Picea abies and Betula pendula at two forest sites
[J]. Biol. Fertil. Soils, 33:17～ 24.
[33] Rajendran N, Matsuda O, Imamura N, et al. 1992. Variation in microbial biomass and community in the sediments of eutrophic bays as described by phospholipid ester-linked fatty acids[J].Appl . Environ. Microbiol . , 58: 562～ 571.
[34] Ringelberg DB, Sutton S, White DC. 1997. Biomass, bioactivity and biodiversity: microbial ecology of the deep subsurface: An alysis of ester-linked phospholipid fatty acids[J]. FEMS Microbiol. Rev., 20:371 ～377.
[35] Robie J.1989. Lipid analysis in microbial ecology[J].BioScience,39(8) :535～541.
[36] Roslev P, Iversen N, Henriksen K. 1998. Direct fingerprinting of metabolically active bacteria in environmental samples by substrate specific radiolabelling and lipid analysis[J]. J. Microbiol.Methods, 31: 99～ 111.
[37] Smith CA, Phiefer CB, Macnaughton SJ, et al. 2000. Quantitative lipid biomarker detection of unculturable microbes and chlorine exposure in water distribution system biofilms [J]. Wat.Res., 34 (10): 2683 ～ 2688.
[38] Smith GA, Nickels JS, Kerger BD, et al. 1986. Quantitative characterization of microbial biomass and community structure in subsurface material:A Procaryotic consortium responsive to organic contamination[J]. Can. J. Microbiol., 32:104 ～ 111.
[39] Tunlid A, Barid BH, Trexler MB, et al. 1985. Determination of phospholipid ester-linked fatty acid and polyβ-hydroxybuty rate for the stimulation of bacterial biomass and activity in the rhizosphere of the rape plant B rassicanapus[J]. Can. J. Microbiol.,31:1113～1119.
[40] Tunlid A, White DC. 1992. Biochemical analysis of biomass community structure, nutritional status and metabolic activity of microbial communities in soil[J]. Soil Biochem, 7:229～262.
[41] Vestal JR, White DC. 1989. Lipid analysis in microbial ecology quantitative approaches to the study of microbial communities[J]. Biosscience, 39: 535 ～ 541.
[42] White DC, Davis WM, Nickels JS, et al. 1979. Determination of the sedimentary microbial biomnss by extractable lipid phosphate[J]. Oecologia, 41: 51 ～ 62.