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连续深松对黑土水热酶特性及细菌群落的影响

张博文1,杨彦明1,李金龙2,陈新宇3,张兴隆1,徐忠山1,刘景辉1*   

  1. (1内蒙古农业大学农学院, 呼和浩特 010019;2阿荣旗农业技术推广中心, 内蒙古呼伦贝尔 162750;3扎赉特旗农业技术推广中心, 内蒙古兴安盟 137600)
  • 出版日期:2018-11-10 发布日期:2018-11-10

Effects of continuous subsoiling on temperature, water content, enzyme activity and bacterial community in black soil.

ZHANG Bo-wen1, YANG Yan-ming1, LI Jin-long2, CHEN Xin-yu3, ZHANG Xing-long1, XU Zhong-shan1, LIU Jing-hui1*   

  1. (1Inner Mongolia Agricultural University College of Agriculture, Hohhot 010019, China; 2Arun Banner Agricultural Technology Promotion Center, Hulunbeir 162750, Inner Mongolia, China; 3Jalaid Banner Agricultural Technology Promotion Center, Hinggan League 137600, Inner Mongolia, China).
  • Online:2018-11-10 Published:2018-11-10

摘要: 为阐明连续深松措施对黑土水热酶活性动态变化及细菌群落多样性的影响,于2016—2017年设置旋耕(CK)、浅松1年(QS1)、浅松2年(QS2)、深松1年(SS1)、深松2年(SS2)、超深松1年(CS1)、超深松2年(CS2)7个处理,研究土壤水分、温度、过氧化氢酶、蔗糖酶、脲酶活性及细菌群落结构变化。结果表明:深松可显著降低0~20 cm土壤含水量,以SS1、SS2降幅最小,提高各处理20~40 cm土壤含水量,以QS2效果最佳,深松2年土壤含水量高于深松1年;各深松处理开花期土壤温度显著低于CK,而小喇叭口期、抽雄期、成熟期则显著高于CK。随着深松深度增加,0~20 cm土壤温度显著降低;深松可维持0~20 cm土壤酶活性长效性,提高20~40 cm土壤酶活性;深松较CK提高0~20 cm土层玉米根际土壤细菌群落丰富度与多样性,深松2年各处理细菌群落丰富度高于深松1年,各深松处理以SS2效果最优。深松可提高酸杆菌门、芽单胞菌门、浮霉菌门、奇古菌门等相对丰度,降低放线菌门、厚壁菌门等具有致病菌种菌门相对丰度。深松可优化土壤水热环境,维持土壤酶活性高效、稳定,协调细菌群落结构构建,促进黑土地可持续利用。

关键词: 城市森林, 梯度分析, 优化对策, 景观生态格局

Abstract: To clarify the effects of subsoiling on the dynamics of soil hydrothermal conditions, soil enzyme activity and bacterial community diversity in black soil, seven treatments were set up from 2016 to 2017, including rotary tillage (CK), one year of surface tillage (QS1), two years of surface tillage (QS2), one year of deep subsoiling (SS1), two years of deep subsoiling (SS2), one year of extradeep subsoiling (CS1), and two years of extradeep subsoiling (CS2). The variations of soil water content, temperature, catalase activity, saccharase activity, urease activity and bacterial community structure among different treatments were assessed. The results showed that subsoiling significantly reduced soil water content of 0-20 cm layer, with SS1 and SS2 showing the lowest reduction, and improved soil water content of 20-40 cm layer, with the highest enhancement in QS2. Soil water content in subsoiling for two years was higher than that in subsoiling for one year. The soil temperature of subsoiling treatments was significantly lower than that of CK at the flowering stage, while soil temperature at jointing stage, tasseling stage, mature stage was significantly higher compared to CK. Soil temperature declined significantly with the increases of subsoiling depth. Subsoiling could maintain longterm soil enzyme activity in 0-20 cm soil layer, and increase soil enzyme activity in 20-40 cm soil layer. Subsoiling improved the richness and diversity of bacterial communities in the rhizosphere soil of 0-20 cm layers. The richness of bacterial communities in subsoiling for two years was higher than that for one year, with SS2 being the best. Subsoiling increased the relative abundance of Acidobacteria, Gemmatimonadetes, Planctomycetes and Thaumarchaeota, but reduced that of Actinobacteria and Firmicutes. Our results indicated that subsoiling can optimize soil hydrothermal environment, maintain high and stable soil enzyme activity, coordinate the structure of bacterial community, and promote the sustainable use of black soil.

Key words: landscape ecological pattern, optimization strategy, urban forest, gradient analysis