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入侵植物粗毛牛膝菊种群遗传多样性及遗传分化

李晓春1,齐淑艳1,2**,姚静1,杨柳1   

  1. (1沈阳大学生命科学与工程学院, 沈阳 110044; 2辽宁省城市有害生物治理与生态安全重点实验室, 沈阳 110044)
  • 出版日期:2015-12-10 发布日期:2015-12-10

Genetic diversity and differentiation of invasive plant Galinsoga quadriradiata populations in China.

LI Xiao-chun1, QI Shu-yan1,2**, YAO Jing1, YANG Liu1   

  1. (1College of Biological Science and Engineering, Shenyang University, Shenyang 110044, China; 2Liaoning Key Laboratory of Urban Integrated Pest Management and EcoSecurity, Shenyang 110044, China)
  • Online:2015-12-10 Published:2015-12-10

摘要: 利用ISSR分子标记技术,分析粗毛牛膝菊(Galinsoga quadriradiata)在中国分布的18个地理种群遗传多样性和遗传结构特点,为粗毛牛膝菊的防控奠定基础。结果表明:筛选的10条引物共扩增出349个位点,经Popgene软件分析,粗毛牛膝菊种群多态位点百分比(P)为8.88%~29.51%,Nei基因多样性指数(H)为0.0248~0.1023;种群遗传分化系数(Gst)为0.4663,种群间基因流(Nm)为0.5723;遗传相似度平均为0.9404,遗传距离平均为0.0621,UPGMA聚类分析将18个粗毛牛膝菊地理种群分为5大类;经Mantel检验,不同地理种群遗传距离与地理距离相关性不显著。研究表明,粗毛牛膝菊种群的遗传多样性较低;各地理种群间产生了一定程度的遗传分化;粗毛牛膝菊的入侵及扩散途径以人为多次反复传播为主。

关键词: 春玉米, 耗水特性, 交替地下滴灌, 产量, 水分利用效率

Abstract: The genetic diversity and genetic structure of 18 Galinsoga quadriradiata populations in China were analyzed by intersimple sequence repeat (ISSR), to lay a foundation for its control. The results showed that 10 primer groups were screened and 349 loci were gotten by ISSR marker analysis. Analyzed by Popgene software, the percentage of polymorphic loci (P) was 8.88%-29.51%, the Nei’s gene diversity index (H) among populations was 0.0248-0.1023, coefficient of gene differentiation (Gst) among populations was 0.4663, gene flow (Nm) among populations was 0.5723, genetic similarity among populations was 0.9404 and genetic distance among populations was 0.0621. The 18 G. quadriradiata populations were clustered into 5 groups by UPGMA cluster analysis. The Mantel test suggested that there was no significant correlation between the genetic distance and geographic distance of different G. quadriradiata populations. In summary, our results suggested that there existed a lower level of genetic diversity and higher level of genetic differentiation of G. quadriradiata populations. The spread of G. quadriradiata in the studied regions was mainly attributed to the events of humanmediated longdistance and repeated multiple dispersal of seeds.

Key words: alternate subsurface drip irrigation, water consumption characteristics, water use efficiency., spring maize, yield