转双价基因抗虫棉对土壤微生物群落多样性的影响

生态学杂志 ›› 2012, Vol. 31 ›› Issue (10): 2486-2492.

转双价基因抗虫棉对土壤微生物群落多样性的影响

乌兰图雅^1,2,3,赵建宁^1,2,李刚^1,2,红梅³,刘红梅^1,2,王慧^1,2,杨殿林^1,2**

(¹农业部环境保护科研监测所，农业部转基因生物生态环境安全监督检验测试中心，天津 300191； ²中国农业科学院武清转基因生物农田生态系统影响野外科学观测试验站，天津 301701； ³内蒙古农业大学生态环境学院，呼和浩特 010018)

出版日期:2012-10-10 发布日期:2012-10-10

Effects of transgenic Bt+CpTI insect-resistant cotton on microbial community diversity in rhizosphere soil.

WULAN Tu-ya^1,2,3, ZHAO Jian-ning^1,2, LI Gang^1,2, HONG Mei³, LIU Hong-mei^1,2, WANG Hui^1,2, YANG Dian-lin^1,2**

(¹AgroEnvironmental Protection Institute, Ministry of Agriculture, Ecosafety Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Tianjin 300191, China; ²Wuqing Experiment Station for Field Observation of Farmland Ecosystem Impact of Genetically Modified Organisms, Chinese Academy of Agricultural Sciences, Tianjin 301701, China; ³ College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

Online:2012-10-10 Published:2012-10-10

摘要/Abstract

摘要： 采用Biolog技术，研究了不同生育期（30、60、90和120 d）转双价(Bt+CpTI)基因抗虫棉SGK321及其亲本非转基因常规棉石远321根际土壤微生物群落多样性变化。结果表明，反映土壤微生物活性的平均颜色变化率(AWCD)呈现出以下变化规律：与亲本常规棉相比，整个培育过程中转双价基因抗虫棉AWCD值除30 d显著高于亲本常规棉(P<0.05)，其他3个时期均显著低于亲本常规棉(P<0.05)。两种棉花根际土壤微生物群落丰富度指数和优势度指数随棉花生育期的不同而有所不同，4个时期转双价基因抗虫棉均匀度指数与亲本常规棉无显著差异。主成分分析结果表明，转双价基因抗虫棉30 d、亲本常规棉60、120 d土壤微生物群落碳源利用方式相似，转双价基因抗虫棉60、90、120 d与亲本常规棉30 d土壤微生物群落碳源利用方式相似。土壤微生物利用的主要碳源为糖类、氨基酸类、羧酸类和聚合物。

关键词: 根序, 细根特征, 季节动态, 杨树人工林, 生产力衰退

Abstract: An experiment with transgenic Bt+CpTI cotton SGK321 and its parental isoline Shiyuan 321 was conducted to examine the effects of planting transgenic Bt+CpTI cotton on the soil microbial community diversity. Rhizosphere soil was sampled at different growth stages (30, 60, 90, and 120 d) of SGK321 and Shiyuan 321. The Biolog analysis showed that the average well color development (AWCD, reflecting soil microbial activity) of all carbon sources changed in the following patterns. As compared with that for Shiyuan 321, the AWCD for SGK321 was significantly higher (P<0.05) at the growth stage of 30 d but significantly lower at other growth stages (P<0.05). The Shannon richness index and Simpson’s dominance index of the soil microbial community changed differently during the growth of SGK321 and Shiyuan 321, whereas the evenness index had no significant difference at the four growth stages of the two cultivars. Principal component analysis indicated that at the SGK321 growth stage of 30 d and the Shiyuan 321 growth stages of 60 and 120 d, and also, at the SGK321 growth stages of 60, 90, and 120 d and the Shiyuan 321 growth stage of 30 d, the carbon sources utilization of soil microbial community was similar. The main carbon sources utilized by the soil microbes were carbohydrates, amino acids, carboxylic acids, and polymers.

Key words: root order, fine root trait, seasonal dynamics, poplar plantation, productivity decline.

乌兰图雅1,2,3,赵建宁1,2,李刚1,2,红梅3,刘红梅1,2,王慧1,2,杨殿林1,2**. 转双价基因抗虫棉对土壤微生物群落多样性的影响[J]. 生态学杂志, 2012, 31(10): 2486-2492.

WULAN Tu-ya1,2,3, ZHAO Jian-ning1,2, LI Gang1,2, HONG Mei3, LIU Hong-mei1,2, WANG Hui1,2, YANG Dian-lin1,2**. Effects of transgenic Bt+CpTI insect-resistant cotton on microbial community diversity in rhizosphere soil.[J]. cje, 2012, 31(10): 2486-2492.

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