生物表面活性剂产生菌的筛选及表面活性剂稳定性研究

生态学杂志 ›› 2005, Vol. ›› Issue (6): 631-634.

生物表面活性剂产生菌的筛选及表面活性剂稳定性研究

牛明芬^1,2, 李凤梅², 韩晓日¹, 郭书海², 牛之欣², 冷延慧², 张春桂²

1. 沈阳农业大学土地与环境学院, 沈阳, 110161;
2. 中国科学院沈阳应用生态研究所, 沈阳, 110016

收稿日期:2004-10-20 修回日期:2005-01-07 出版日期:2005-06-10
通讯作者: 郭书海
基金资助:
国家重点基础研究发展规划项目(2004CB418500);国家高技术研究发展计划前沿探索课题(2004AA649060);中国科学院沈阳应用生态研究所知识创新工程资助项目(SLYQY0401).

Isolation of biosurfactant-producing microorganisms and their stability

NIU Mingfen^1,2, LI Fengmei², HAN Xiaori¹, GUO Shuhai², NIU Zhixin², LENG Yanhui², ZHANG Chungui²

1. College of Land and Environment, Shenyang Agricultural University, Shenyang 110016, China;
2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China

Received:2004-10-20 Revised:2005-01-07 Online:2005-06-10

摘要/Abstract

摘要： 大庆油田油泥样品经富集培养,平板分离,获得52株菌.排油性实验和表面张力测定表明,菌株B₂₂、B₂₄、B₂₅产生的表面活性剂表面活性稳定,表面张力较低.温度、pH和NaCl浓度实验证实,细菌B₂₂产生的生物表面活性剂可耐受120℃高温,另2种生物表面活性剂可耐受80℃;3种细菌生物表面活性剂对pH有广泛适应性,B₂₂ pH适应范围为.0～13.0,B₂₄、B₂₅的pH适应范围为2.0～13.0;Na-Cl浓度对表面活性剂的生物活性影响不大.将3株菌的生物表面活性剂用于室内油泥处理实验,72 h石油去除率达70%以上.

关键词: 农业景观, 生态建设, 水土保持, 遥感和地理信息系统

Abstract: 52 biosurfactant-producing microorganisms were isolated with the methods of enrichment culture and plate cultivation from oil sludge of Daqing Oil Field.Experiments of oil displacement activity and surface tension showed that the three strains (including B₂₂,B₂₄ and B₂₅) had high surface activity and low surface tension.The analysis of physico-chemical properties indicated that the biosurfactant produced by B₂₂ could bear high temperature up to 120 ℃,while the other two could bear 80 ℃.The three biosurfactants had a wide adaptability to pH.B₂₂ had a wide adaptable pH range of 4.0～13.0,while that of B₂₄ and B₂₅ was 2.0～13.0.NaCl concentration had no obvious effect on the biological activity of the biosurfactants.Employing the 3 strains biosurfactants to treat oil sludge,more than 70% of the oil was removed after 72 h.

Key words: Agricultural landscape, Ecological construction, Soil and water conservation, Remote sensing and GIS

中图分类号:

Q935

牛明芬, 李凤梅, 韩晓日, 郭书海, 牛之欣, 冷延慧, 张春桂. 生物表面活性剂产生菌的筛选及表面活性剂稳定性研究[J]. 生态学杂志, 2005, (6): 631-634.

NIU Mingfen, LI Fengmei, HAN Xiaori, GUO Shuhai, NIU Zhixin, LENG Yanhui, ZHANG Chungui. Isolation of biosurfactant-producing microorganisms and their stability[J]. cje, 2005, (6): 631-634.

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