不同寄主源栎实象共生细菌多样性

doi:10.13292/j.1000-4890.202502.041

生态学杂志 ›› 2025, Vol. 44 ›› Issue (2): 460-470.doi: 10.13292/j.1000-4890.202502.041

不同寄主源栎实象共生细菌多样性

柳璎珊^1,2,应玥¹,彭嫔嫔³,骆剑锋⁴,李志红¹,张威¹,舒金平^1*

（¹中国林业科学研究院亚热带林业研究所，杭州 311400； ²南京林业大学，南京 210037； ³浙江林信林业技术服务有限公司，杭州 311400； ⁴杭州富阳国有农林资产经营有限公司，杭州 311400）

出版日期:2025-02-10 发布日期:2025-02-10

The symbiotic bacterial diversity of Curculio spp. from different hosts.

LIU Yingshan^1,2, YING Yue¹, PENG Pinpin³, LUO Jianfeng⁴, LI Zhihong¹, ZHANG Wei¹, SHU Jinping^1*

(¹Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China; ²Nanjing Forestry University, Nanjing 210037, China; ³Zhejiang Linxin Forestry Technology Service Co., Hangzhou 311400, China; ⁴Hangzhou Fuyang State-owned Agricultural and Forestry Assets Management Co., Hangzhou 311400, China).

Online:2025-02-10 Published:2025-02-10

摘要/Abstract

摘要： 为进一步揭示共生细菌在栎实象寄主适应中的作用，分析比较了两种栎实象取食不同寄主植物时其共生细菌群落组成与结构的变化。本研究选取取食多穗石柯（Lithocarpus polystachyus）、钩锥（Castanopsis tibetana）和苦槠（Castanopsis sclerophylla）的二斑栎实象（多寄主）（Curculio bimaculatus）和仅取食钩锥的栗实象（专寄主）（Curculio davidi）两种栎实象，利用Illumina二代测序技术对其幼虫体内共生细菌的16S rRNA V3-V4区序列进行高通量测序，并基于OTU进行群落Alpha、Beta多样性分析与差异比较分析。经过Illumina质控，共得到919980条优化序列，分属914个OTU，分布在24个门，53个纲，129个目，219个科，400个属。变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）、放线菌门（Actinobacteriota）在各组样品中均占优势。取食不同植物的栎实象共生细菌Alpha多样性差异显著，而Beta多样性分析显示群落结构较为相似。取食钩锥的两种栎实象中均未发现沃尔巴克氏体属（Wolbachia）、欧文氏菌属（Erwinia），Alpha多样性无显著差异，但栗实象中缺乏短小杆菌属（Curtobacterium）、链霉菌属（Streptomyces）、红球菌属（Rhodococcu），且伴蝇菌属（Sodalis）和立克次体属（Rickettsia）丰度极低。研究表明，取食不同植物对栎实象的共生细菌的多样性与丰富度影响较大，而栎实象的种类则主要影响共生细菌的群落结构。

关键词: 共生细菌, 栎实象, 16S rRNA, 群落结构, 寄主适应

Abstract: The aims of this study were to reveal the diversity and population differences of symbiotic bacteria of weevils (Curculio spp.) from different host sources, to explore the effects of different host plants on symbiotic bacteria of weevils, and to facilitate further research on the roles of symbiotic bacteria in promoting weevil host adaptation. Two species of weevil were examined, Curculio bimaculatus (multiple hosts), which feeds on Lithocarpus polystachyus, Castanopsis tibetana and Castanopsis sclerophylla, and Curculio davidi (specialized host), which feeds only on Castanopsis tibetana. High-throughput sequencing of the 16S rRNA V3-V4 region sequences of the symbiotic bacteria of weevil larvae was performed using Illumina technology. Community alpha and beta diversity based on OTU (operational taxonomic unit) were calculated. After Illumina quality control, a total of 919980 high quality sequences were obtained, which belonged to 914 OTUs in 24 phyla, 53 classes, 129 orders, 219 families and 400 genera. Proteobacteria, Firmicutes, Actinobacteriota were dominant in all four groups of samples. The alpha diversity of symbiotic bacteria differed between the weevils feeding on different plants, but the community structure was similar as indicated by beta diversity index. Wolbachia and Erwinia were not found in both weevils feeding on Castanopsis tibetana, without significant difference in their alpha diversity. Curtobacterium, Streptomyces and Rhodococcu were lacking in Curculio davidi, and the abundance of Sodalis and Rickettsia was very low. The diversity and abundance of symbiotic bacteria were strongly influenced by plant species that the weevils fed on, while the species of the weevils mainly influenced community structure of symbiotic bacteria. This study may provide a reference for further investigation of the role of symbiotic bacteria in driving the differentiation and adaptation of weevils’ hosts.

Key words: symbiotic bacteria, Curculio spp., 16S rRNA, community structure, host adaptation

柳璎珊, 应玥, 彭嫔嫔, 骆剑锋, 李志红, 张威, 舒金平. 不同寄主源栎实象共生细菌多样性[J]. 生态学杂志, 2025, 44(2): 460-470.

LIU Yingshan, YING Yue, PENG Pinpin, LUO Jianfeng, LI Zhihong, ZHANG Wei, SHU Jinping. The symbiotic bacterial diversity of Curculio spp. from different hosts.[J]. Chinese Journal of Ecology, 2025, 44(2): 460-470.

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不同寄主源栎实象共生细菌多样性

The symbiotic bacterial diversity of Curculio spp. from different hosts.

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