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生态学杂志 ›› 2020, Vol. 39 ›› Issue (8): 2593-2601.

• 研究报告 • 上一篇    下一篇

宁夏枸杞叶不同生长时期内生细菌群落动态及其影响因素

苟琪,吕燕,张涛,李靖宇,赵会君,刘建利*   

  1. (北方民族大学生物科学与工程学院, 国家民委生态系统建模和应用重点实验室, 银川 750021)
  • 出版日期:2020-08-10 发布日期:2021-02-10

Dynamics and influencing factors of endophytic bacterial community in leaves of Lycium barbarum during different growth periods.

GOU Qi, LYU Yan, ZHANG Tao, LI Jing-yu, ZHAO Hui-jun, LIU Jian-li*   

  1. (College of Biological Sciences and Engineering, North Minzu University, Key Laboratory of Ecosystem Modelling and Application, State Ethnic Affairs Commission, Yinchuan 750021, China).
  • Online:2020-08-10 Published:2021-02-10

摘要: 为探究宁夏枸杞叶内生细菌群落随生长变化动态及其影响因素,利用高通量测序技术分析宁夏枸杞幼叶、成熟叶和老叶3个典型生长时期叶内生细菌群落,并通过典范对应分析和方差分解分析探究气候因子代表的宿主植物生活环境、叶光合参数代表的宿主器官生理状态、叶内微生物生长可利用营养物质代表的植物体内内生菌生存微环境等三类环境因子对叶内生菌群落动态变化的影响。结果表明:3个宁夏枸杞叶生长时期内生细菌共28个门、52个纲、118个目、249个科、503个属、738个种、978个OTUs,α多样性及丰富度以幼叶中最多,随着叶片生长而减少,至叶片成熟老化,保持在较低的水平;变形菌门γ变形菌纲是3个生长时期枸杞叶片中的优势类群,但幼叶内生细菌中优势科属为黄单胞菌科食单胞菌属,成熟叶内生细菌优势科属为假单胞菌科假单胞菌属,老叶内生细菌优势科属为肠杆菌科泛菌属;成熟叶和老叶内生细菌OTU以继承前一个生长时期内生细菌OTU为主;叶内生细菌功能随叶片生长时期改变发生细微变化;不同生长时期影响叶内生细菌群落组成的环境因子不同,以叶内微生物生长可利用营养物质对宁夏枸杞叶内生细菌群落组成影响最大;宁夏枸杞叶内生细菌从各群落特征对不同叶生长时期内外环境变化做出响应,植物体内内生菌生存微环境是影响宁夏枸杞内生菌群落动态变化主要因素。

关键词: 内生细菌, 群落特征, 环境因子

Abstract: To explore dynamics and influencing factors of endophytic bacterial community in leaves of Lycium barbarum at different growth stages, endophytic bacterial community in young, mature and old leaves of Lycium barbarum were examined using the high-throughput sequencing. Canonical correspondence analysis and variance partitioning canonical correspondence analysis were used to analyze the effects of three environmental factors on the dynamics of endophytic bacteria community, including the living conditions of host plants represented by climatic factors, the physiological status of host plant’s organs represented by photosynthesis parameters, and the endophytic bacteria living micro-environmental condition in host plants represented by nutrients that can be utilized by microorganisms. The results showed that 28 phyla, 52 classes, 118orders, 249 families, 503 genera, 738 species and 978 OTUs of endophytic bacteria were found during three growth periods. The alpha diversity and richness of OTU were the highest in the young leaves, decreased with the ages of leaves, and being at a relatively low level in mature and old leaves. The class γProteobacteria of phylum Proteobacteria dominated the endophytic bacteria in the leaves. In addition, genus Stenotrophomonas in family Xanthomonas, genus Pseudomonas in family Pseudomonadaceae, and genusPantoea in family Enterobacter were dominant in young, mature, and old leaves, respectively. The endophytic bacteria OTUs in mature and old leaves were mainly inherited from the preceding growth period. The function of endophytic bacteria changed slightly with the growth stage of leaves. The environmental factors affecting the composition of endophytic bacterial community differed across growth stages, with foliar nutrients having the greatest impact. The endophytic bacteria in leaves of Lycium barbarum responded to the changes of in vivo and in vitro environment during different growth stages based on each characteristic of endophytic bacterial community. The living micro-environmental condition of endophytic bacteria in host plants was the main factor affecting the dynamics of endophytic bacterial community in leaves of Lycium barbarum.

Key words: endophytic bacteria, community characteristics, environmental factor.