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• 方法与技术 • 上一篇    

西藏牦牛遗传多样性的ISSR分析

姚慧1,钟金城1,姬秋梅2,张成福2,信金伟2,陈智华1**,柴志欣1   

  1. (1西南民族大学动物遗传育种学国家民委教育部重点实验室, 成都 610041; 2西藏自治区农牧科学院畜牧兽医研究所, 拉萨 850000)
  • 出版日期:2015-11-10 发布日期:2015-11-10

Analysis of ISSR genetic polymorphism for Tibetan yak.

YAO Hui1, ZHONG Jin-cheng1, JI Qiu-mei2, ZHANG Cheng-fu2, XIN Jin-wei2, CHEN Zhi-hua1**, CHAI Zhi-xin1   

  1. (1Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu 610041, China; 2Institute of Animal Science and Veterinary, Tibetan Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China)
  • Online:2015-11-10 Published:2015-11-10

摘要: 选用10对ISSR引物对西藏地区17个牦牛类群(或品种)共850头牦牛进行了PCR扩增,利用Popgen 32软件对数据进行统计分析。结果表明:10对ISSR引物共扩增出134条条带,其中多态性条带132条,多态性比率为95%,扩增片段的大小范围在200~2000 bp。17个牦牛类群的平均有效等位基因数为1.498,Nei遗传多样性指数平均值为0.294,平均Shannon多样性指数为0.449,各位点遗传多样性存在一定差别,说明17个西藏牦牛类群具有丰富的遗传多样性。聚类结果表明,西藏牦牛大致分为3类:错那牦牛、江达牦牛、工布江达牦牛、康布牦牛、巴青牦牛、类乌齐牦牛、桑桑牦牛、嘉离牦牛、斯布牦牛和隆子牦牛聚为1类;丁青牦牛、聂荣牦牛、帕里牦牛、日多牦牛、桑日牦牛和申扎牦牛聚为1类;仲巴牦牛单独聚为1类。聚类分析结果与牦牛的地理分布大体一致。

关键词: 农用地毯, 苹果, 覆盖, 硝酸盐代谢, 根区土壤, 稻草苫

Abstract: In order to analyze the genetic diversity of Tibetan yaks, 10 pairs of primers were selected from ISSR primer library and used to analyze 17 groups (species), with a total number of 850 yaks, by PCR amplification. The obtained statistical data were also analyzed by Popgen 32 software. The results showed that 10 pairs of ISSR primers amplified a total of 134 bands including 132 polymorphic bands. The polymorphism ratio is 95% and the size of the amplified fragment ranges from 200 to 2000 bp. The average effective number of alleles of 17 yak groups is 1.498, the mean index of Nei’s genetic diversity is 0.294 and average Shannon diversity index is 0.449. Clustering results show that Tibetan yaks are roughly divided into three categories: The first group includes Cuona yak, Jiangda yak, Gongbujiangda yak, Kangbu yak, Baqing yak, Leiwuqi yak, Sangsang yak, Jiali yak, Sibu yak and Longzi yak; the second includes Dingqing yak, Nierong yak, Pali yak, Riduo yak, Sangri yak and Shenza yak; the third group only includes Zhongba yak. Cluster analysis results are basically consistent with the geographic distribution of yaks.

Key words: straw mat, agricultural carpet, root-zone soil, nitrate metabolizing., apple, mulching