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赤霉素浸种时长和施用浓度对重度干旱和盐胁迫下黄芪幼苗发育的影响

王楠1,高静2,黄文静1,李铂1,何懿菡1,唐志书1*,宋忠兴1   

  1. 1陕西中医药大学陕西省中药资源产业化协同创新中心, 陕西咸阳 712083;2陕西中医药大学药学院, 陕西咸阳 712046)
  • 出版日期:2019-09-10 发布日期:2019-09-10

The effects of concentration and duration of seed soaking by GA3 on the development of Astragalus membranaceus seedlings under severe drought and salt stresses.

WANG Nan1, GAO Jing2, HUANG Wen-jing1, LI Bo1, HE Yi-han1, TANG Zhi-shu1*, SONG Zhong-xing1   

  1. (1ShaanxiCollaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, Shaanxi, China; 2College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi, China).
  • Online:2019-09-10 Published:2019-09-10

摘要: 为研究赤霉素(GA3)浸种时长和施用浓度对重度干旱(PEG)和盐(NaCl)胁迫下膜荚黄芪(Astragalus membranaceus)种子幼苗生长的影响,使用5个浓度(0、50、100、200和400 mg·L-1)的GA3将种子浸泡5个时长(6、12、24、36和48 h)后置入渗透势为-0.6 MPa的PEG和-0.9 MPa的NaCl溶液中进行发芽试验,测定最终发芽率(TGP)、平均发芽时间(MGT)、根/芽抑制指数(R/SII)、芽长(SL)、根长(RL)、芽干重(SDW)和根干重(RDW)等数据。结果表明:随着延长浸种时间和增加GA3浓度,TGP和MGT在两种胁迫下分别呈现上升和缩短的趋势;NaCl胁迫下,400 mg·L-1 GA3浸种超过36 h对SL起到显著抑制作用,而对RL的显著抑制则在200 mg·L-1 GA3及以上浓度浸种36 h和400 mg·L-1 GA3浸种24 h开始出现(P<0.05);PEG胁迫下,对SL和RL的显著抑制均是在200 mg·L-1 GA3及以上浓度浸种36 h开始出现(P<0.05);NaCl胁迫下,对SDW和RDW的显著抑制均是在200 mg·L-1 GA3及以上浓度浸种36 h开始出现,而PEG胁迫下对SDW和RDW的显著抑制均是在200 mg·L-1 GA3及以上浓度浸种48 h开始出现(P<0.05);TGP、MGT、SL、RL、SDW和RDW之间存在显著的相关性(P<0.05)。利用Design Expert软件预测分析得出:施用103 mg·L-1 GA3浸种40 h和51 mg·L-1 GA3浸种48 h分别是在NaCl和PEG胁迫下能够有助于黄芪萌发和苗期发育的最优化处理组合方式。

关键词: 植被恢复, 生长阶段, 土壤微生物生物量碳, 土壤理化性质

Abstract: An experiment was conducted to explore the effects of soaking time and applied level of gibberellic acid (GA3) on the development of Astragalus membranaceus seedlings under severe drought and salt stresses. There were five application levels (0, 50, 100, 200 and 400 mg·L-1) of GA3 and five duration periods (6, 12, 24, 36 and 48 h) for seed soaking, After that, the treated seeds were transferred to -0.6 and -0.9 MPa NaCl solutions respectively. Total germination percentage (TGP), mean germination time (MGT), root/shoot inhibition index (R/SII), shoot length (SL), root length (RL), shoot dry weight (SDW) and root dry weight (RDW) were measured. The results showed that, with the extension of soaking time and the increase of GA3 application levels, TGPs of A. membranaceus were enhanced under both stresses while MGTs were substantially shortened. SL of soaking seeds with 400 mg·L-1 GA3 for more than 36 h under NaCl stress was significantly inhibited, while RL was significantly reduced at the level of 200 mg·L-1 GA3 or higher for 36 h and 400 mg·L-1 GA3 for 24 h (P<0.05). The significant inhibition of SL and RL under PEG stresses began at 36 h after soaking seeds at 200 mg·L-1 GA3 or higher level (P<0.05). The significant inhibition of GA3 on SDW and RDW under NaCl stress began at 36 h after soaking seeds at 200 mg·L-1 or higher, while the significant inhibition of GA3 on SDW and RDW under PEG stress began at 48 h after soaking seeds at 200 mg·L-1 GA3 or higher (P<0.05). There were significant correlations among TGP, MGT, SL, RL, SDW and RDW (P<0.05). The optimum treatment combinations of 103 mg·L-1 GA3 for 40 h and 51 mg·L-1 GA3 for 48 h could contribute to the germination and seedling development under NaCl and PEG stresses, respectively.

Key words: vegetation restoration, growth stage, soil microbial biomass carbon, soil physicalchemical property.