欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 方法与技术 • 上一篇    下一篇

鸟类血液寄生虫研究方法的发展与比较

周婕1,夏灿玮1,臧春鑫2,董路1*
  

  1. (1北京师范大学生命科学学院, 生物多样性与生态工程教育部重点实验室, 北京 100875; 2中国环境科学研究院, 生物多样性研究中心, 北京 100012)
  • 出版日期:2016-08-10 发布日期:2016-08-10

Progress and comparison of techniques in the study of avian blood parasites.

ZHOU Jie1, XIA Can-wei1, ZANG Chun-xin2, DONG Lu1*   

  1. (1Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China; 2Biodiversity Research Center, Chinese Research Academy of Environmental Science, Beijing 100012, China).
  • Online:2016-08-10 Published:2016-08-10

摘要: 鸟类血液寄生虫是研究寄生虫疾病以及物种进化的一个重要模式类群。本文在回顾鸟类血液寄生虫检测历史的基础上,比较了血涂片检测方法与分子生物学检测方法在样品制备、感染率检测的灵敏度以及寄生虫分类的准确性等方面存在的差异,提出需根据研究目的选择适当的检测方法的建议,以准确掌握鸟类血液寄生虫的感染情况。通过利用Meta分析对两类检测方法进行比较,发现分子方法检测到的鸟类血液寄生虫感染率显著高于镜检方法,当感染率低时镜检方法会低估感染率。本文还展望了分子生物学技术对鸟类血液寄生虫研究的推动作用。

关键词: 大豆, 干旱, &alpha, -萘乙酸, 抗氧化系统

Abstract: Avian blood parasites are an important model group in the study of parasitic disease and species evolution. On the basis of historical overview on detection of avian blood parasites, we compared the differences in sample preparation, sensibility of detecting prevalence and accuracy of parasites classification between blood smear test methods and molecular biology test methods. We proposed that, to exactly obtain the prevalence of avian blood parasites, it is important to choose test methods based on the research purpose. Metaanalysis indicated that the detection rate of molecular biology method was significantly higher than that of blood smear method. Moreover, blood smear method might underestimate the prevalence when it was low. This article also forecasted the role of molecular technique in promoting the studies of avian blood parasites.

Key words: soybean, drought stress, NAA, antioxidant system.