祁连山天然气水合物赋存区钻孔细菌多样性

生态学杂志

祁连山天然气水合物赋存区钻孔细菌多样性

武淑娇¹,韩路¹,吕杰^1**,董建英¹,祝有海²

(¹北京化工大学，北京 100029； ²中国地质科学院矿产资源研究所，北京 100037)

出版日期:2012-12-10 发布日期:2012-12-10

Bacterial diversity in bore holes of gas hydrate-rich deposit districts in Qilian Mountains of Northwest China.

WU Shu-jiao¹, HAN Lu¹, LU Jie^1**, DONG Jian-ying¹, ZHU You-hai²

(¹Beijing University of Chemical Technology, Beijing 100029, China; ²Institute of Mineral Resources, Chinese Academy of Geological Sciences, Beijing 100037, China)

Online:2012-12-10 Published:2012-12-10

摘要/Abstract

摘要： 分析了青海省祁连山冻土区天然气水合物赋存区DK-6钻孔的4个样品，对岩心样品进行处理提取微生物总基因组，采用PCR构建了细菌16S rDNA基因文库，4个文库包括44个OTU，其中有厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、变形杆菌门(Proteobacteria)（包括α-、β-和γ-变形杆菌亚群）、放线菌纲(Actinobacteria)和异常球菌栖热菌门(Deinococcus-Thermus)5类，煤、泥岩、粉砂岩等不同岩性的微生物群落之间显示出较大的组成差异，优势菌不同。细菌菌群多样性随采集点地质环境不同而有较明显的变化，天然气水合物含量、水含量、有机质含量等环境因素对冻土区天然气水合物赋存区中细菌菌群有一定的影响。4个样品中存在的微生物大部分可以代谢有机烃类，在天然气水合物环境的特殊条件下，外界环境因素制约了微生物的种类。

关键词: 外来杂草, 入侵, 种间相互作用, 化感作用, 化学机制

Abstract: In this study, four core samples were collected from the DK-6 bore holes of gas hydrate-rich deposit districts in the permafrost zone of Qilian Mountains, Qinghai Province of Northwest China, with the total bacterial genome in the samples analyzed. Four 16S rDNA gene libraries were created by PCR. There were 44 OTUs, including five categories, i.e., Firmicutes, Bacteroidetes, Proteobacteria (α-, β-, and γ-Proteobacteria), Actinobacteria, and Deinococcus-Thermus, in the four gene libraries. The microbial communities of different lithology showed a relatively large composition difference, and had different dominant species. Lithology played a certain decisive role to the growth of bacteria. Bore holes DK6-12 (117.5 m) and DK6-17 (161.9 m) were of mudstone, in which, Alcaligenes xylosoxidans was the dominant bacterium. K6-23 (194.6 m) was of fine sandstone, in which, a large number of Spirosoma panaciterrae was found in cloning sequences. DK6-25 (213 m) was of coal, with the highest abundance of Pantoea ananatis. Most of the microbes presented in the four samples could metabolize organic hydrocarbon. Our results indicated that in the gas hydrate-rich deposit districts in permafrost zone, environmental factors limited the diversity of microbes.

Key words: Exotic weeds, Invasion, Species interaction, Allelopathy, Chemical mechanism

武淑娇1,韩路1,吕杰1**,董建英1,祝有海2. 祁连山天然气水合物赋存区钻孔细菌多样性[J]. 生态学杂志.

WU Shu-jiao1, HAN Lu1, LU Jie1**, DONG Jian-ying1, ZHU You-hai2. Bacterial diversity in bore holes of gas hydrate-rich deposit districts in Qilian Mountains of Northwest China.[J]. cje.

[1]	池鑫晨,宋超,朱向涛,王楠,王晓雨,白尚斌. 毛竹入侵常绿阔叶林对土壤活性有机碳氮的动态影响 [J]. 生态学杂志, 2020, 39(7): 2263-2272.
[2]	赵孟良,王紫莹,赵文菊,任延靖. 菊芋块茎浸提液对生菜种子萌发生长的化感作用 [J]. 生态学杂志, 2020, 39(7): 2205-2213.
[3]	王桔红，陈文,张燕芳,李云,史生晶,郑佳玉,陈燕霞. 不同入侵程度的微甘菊及本土种豨莶碳氮磷化学计量特征与营养策略 [J]. 生态学杂志, 2020, 39(6): 1994-2003.
[4]	罗莉,李龙沁,许光耀,杨皓璐,贺梦璇,李洪远. 黑、吉、辽、蒙归化植物分布格局及其影响因素 [J]. 生态学杂志, 2020, 39(5): 1492-1500.
[5]	熊韫琦, 赵彩云. 表型可塑性与外来植物的成功入侵 [J]. 生态学杂志, 2020, 39(11): 3853-2864.
[6]	侯新星,辛建攀,陆梦婷,田如男. 江苏外来入侵植物区系、生活型及繁殖特性 [J]. 生态学杂志, 2019, 38(7): 1982-1990.
[7]	张璞进,赵利清,梁晨霞,张国龙,张迁迁,岳丽,徐步云,杨劼,张培青,清华. 内蒙古外来植物入侵风险评价 [J]. 生态学杂志, 2019, 38(7): 1973-1981.
[8]	靳书瑞,罗渡,张海涛,牟希东,胡隐昌. 不同生活史时期福寿螺（Pomacea canaliculata）对酸胁迫的响应 [J]. 生态学杂志, 2019, 38(5): 1426-1432.
[9]	安炎黄,赵启安,陈璐,赵敏,张莉环,杨宁. 磷脂酶参与二萜类化合物冬凌草甲素(oridonin)对拟南芥的化感作用 [J]. 生态学杂志, 2019, 38(4): 995-1003.
[10]	章家恩,郭靖,赵本良,秦钟. 福寿螺的全方位综合防控技术体系构建与开发利用 [J]. 生态学杂志, 2019, 38(12): 3831-3838.
[11]	马超,庄睿花,王时聪,杨欣润,柴如山,王擎运,叶新新,张震,郜红建. 微生物入侵影响因素研究进展 [J]. 生态学杂志, 2019, 38(10): 3195-3201.
[12]	张梅,马可心,唐丽丽,韩建华,郑璞帆,王童童,石福臣. 外来入侵植物反枝苋对本地植物功能性状的影响 [J]. 生态学杂志, 2019, 38(10): 2925-2933.
[13]	许光耀,李洪远,莫训强,孟伟庆. 外来入侵植物管理与控制成效的制约因素 [J]. 生态学杂志, 2019, 38(10): 3169-3176.
[14]	梁碧霞,徐艳萍,武正军,黄锦龙. 鲤鱼对外来入侵物种鳄龟及巴西龟的视觉识别 [J]. 生态学杂志, 2019, 38(1): 205-209.
[15]	全威,王明,桑卫国. 外来入侵植物风传扩散过程模拟模型选择 [J]. 生态学杂志, 2018, 37(9): 2840-2848.