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精胺对荇菜抗氧化酶系汞毒害的缓解作用

王学;徐恒戬   

  1. 山东理工大学生命科学学院, 山东淄博 255049
  • 收稿日期:2008-02-04 修回日期:1900-01-01 出版日期:2008-10-10 发布日期:2008-10-10

Mitigation effects of spermine on Hg injury of Nymphoides peltatum’s antioxidant enzyme system.

WANG Xue;XU Heng-jian   

  1. College of Life Science, Shandong University of Technology, Zibo 255049, Shandong, China
  • Received:2008-02-04 Revised:1900-01-01 Online:2008-10-10 Published:2008-10-10

摘要: 研究了外施不同浓度的精胺(Spm)对15 μmol·L-1汞胁迫下,荇菜(Nymphoides peltatum)叶中SOD、CAT、APX、POD保护酶的活性、O-·2产生速率、MDA、叶绿素、可溶性蛋白、游离态多胺含量的影响。结果表明:外施0.05~0.5 mmol·L-1的Spm显著提高了汞胁迫下荇菜叶内的亚精胺、精胺水平以及叶绿素和可溶性蛋白的含量;并能提高SOD、CAT和APX的活性,降低O-·2的产生速率,减缓MDA的积累;0.05~0.5 mmol·L-1的外源Spm可增强植物抗氧化胁迫的能力,缓解汞对荇菜的毒害作用,其中0.05~0.1 mmol·L-1的外源Spm效果最佳,但当Spm浓度达到5 mmol·L-1时,却加剧了汞的毒害作用。

关键词: CO2, 大棚, 百合, 化感物质

Abstract: This paper studied the effects of different concentration exogenous spermine (Spm) on the activities of antioxidant enzymes (SOD, CAT, POD, APX), generation rate of O-·2, and contents of MDA, free polyamines (PAs), chlorophyll and soluble protein inNymphoides peltatum leaves under 15 μmol·L-1 Hg stress. The results showed that under the Hg stress, 0.05-0.5 mmol·L-1 of exogenous Spm could significantly increase the contents of leaf spermidine (Spd), Spm, chlorophyll, and soluble protein, promote the activities of SOD, CAT, and APX, and decrease the generation rate of O-·2 and the accumulation of MDA. It was suggested that 0.05-0.5 mmol·L-1 of exogenous Spm could enhance the resistance of N. peltatum to oxidant stress, and mitigate the toxic effects of Hg. The optimum dose of exogenous Spm was 0.05-0.1 mmol·L-1, while 5 mmol·L-1 of Spm could aggravate the toxic effects of Hg.

Key words: CO2, Greenhouse, Lily, Allelochemical