欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志 ›› 2021, Vol. 40 ›› Issue (2): 525-533.doi: 10.13292/j.1000-4890.202102.006

• 研究报告 • 上一篇    下一篇

人工湿地对抗生素复合污染的净化效果及微生物群落响应

覃岚倩1,白少元1,2*,张琴2,丁海静1,邓振贵3     

  1. 1桂林理工大学广西矿冶与环境科学实验中心, 广西桂林 541004; 2桂林理工大学广西环境污染控制理论与技术重点实验室, 广西桂林 541004;3广西恒晟水环境治理有限公司, 广西桂林 541004)
  • 出版日期:2021-02-10 发布日期:2021-07-09

Purification effect of constructed wetland on antibiotic compound pollution and the microbial community response.

QIN Lan-qian1, BAI Shao-yuan1,2*, ZHANG Qin2, DING Hai-jing1, DENG Zhen-gui3   

  1. (1Guangxi Experimental Center of Mining, Metallurgy and Environmental Science, Guilin 541004, Guangxi, China; 2Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, Guangxi, China; 3Guangxi Hengsheng Water Environment Management Co. Ltd., Guilin 541004, Guangxi, China).
  • Online:2021-02-10 Published:2021-07-09

摘要: 为研究人工湿地对氟喹诺酮类(FQs)、磺胺类(SFs)、四环素类(TCs)抗生素的净化性能及微生物群落的响应,以运行多年的单层及多层(3层、6层)基质结构的水平潜流人工湿地为对象,分别采用固相萃取高效液相色谱法和高通量测序来检测抗生素含量和微生物菌落结构,分析对比分层结构湿地系统中3类8种抗生素的去除规律、对常规污染物去除的影响以及系统微生物群落结构的变化。结果表明:人工湿地对FQs、磺胺噻唑、磺胺二甲基嘧啶、氧四环素表现出较好的去除效果(>95%),相同条件下,对磺胺嘧啶和四环素去除效果较差(61.9%~66.8%)。多层结构湿地系统对抗生素的平均去除率较单层结构湿地提高3.2%。抗生素的加入使湿地系统对COD、NH4+-N的去除效果略有增加,平均去除率分别提高了18.5%和16.5%,却使TP的平均去除率下降(降低了28.6%)。FQs、SFs和TCs抗生素加入后,3个湿地系统的平均Shannon指数由5.80降到4.66,平均Simpson指数由0.017增加到0.063。主要优势门Proteobacteria和Bacteroidetes不变,其余优势门(Chloroflexi、Firmicutes、Verrucomicrobia和Acidobacteria)主次位置发生改变。优势属结构出现明显变化,反硝化菌AcinetobacterAquabacteriumPseudomonas丰度显著升高,且取代除磷菌DechloromonasFlavobacterium成为主要优势菌属。

 

关键词: 水平潜流人工湿地, 抗生素, 微生物

Abstract: To understand the purification performance and microbial community structureresponse of constructed wetlands to antibiotics additions of fluoroquinolones (FQs), sulfonamides (SFs) and tetracyclines (TCs), we measured the antibiotic contents and microbial community structure in horizontal subsurface flow constructed wetlands with single-layer matrix structure and multi-layer matrix structure (3 and 6 layers) which have been in operation for many years, with solid phase extraction-high performance liquid chromatography and high-throughput sequencing, respectively. We analyzed the removal results of eight antibiotics belonging to three types in wetland systems, the effects on the removal of conventional pollutants, and changes of microbial community structure. The results showed that the constructed wetlands had good removal effects on FQs, sulfathiazole, sulfamethazine and oxytetracycline (>95%), but with relatively poor effects on sulfadiazine and tetracycline (61.9%-66.8%) under the same conditions. The purification effect of multi-layer wetland systems on antibiotics was 3.2% higher than that of single layer wetland system. The addition of antibiotics increased the removal efficiency of COD and NH4+-N by 18.5% and 16.5%, respectively, but decreased that of TP by 28.6%. After the addition of FQs, SFs, and TCs, mean Shannon index in the three wetland systems decreased from 5.80 to4.66, while mean Simpson index increased from 0.017 to 0.063. Before and after the addition of antibiotics, the main dominant phyla (Proteobacteria and Bacteroidetes) remained unchanged, while the primary and secondary positions of the other dominant phyla (Chloroflexi, Firmicutes, Verrucomicrobia, and Acidobacteria) changed. There were obvious changes in the structure of dominant genera. The abundance of denitrifying bacteria Acinetobacter, Aquabacterium and Pseudomonas were significantly increased, replacing the phosphorusremoving bacteria Dechloromonas and Flavobacterium as the main dominant genera.

Key words: horizontal subsurface flow constructed wetland, antibiotics, microbial community.