四环素类抗性基因在黑土农田和蔬菜中的分布特征

doi:10.13292/j.1000-4890.202403.033

生态学杂志 ›› 2024, Vol. 43 ›› Issue (3): 758-765.doi: 10.13292/j.1000-4890.202403.033

四环素类抗性基因在黑土农田和蔬菜中的分布特征

武轶¹,张珣^1*,王宝玉^2,3,高程程^2,3,黎馨月¹,安婧^2*

(¹沈阳大学环境学院，沈阳 110044； ²中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室，沈阳 110016； ³中国科学院大学，北京 100049)

出版日期:2024-03-10 发布日期:2024-03-13

Distribution characteristics of tetracycline resistance genes in black soil farmland and vegetables.

WU Yi¹, ZHANG Xun^1*, WANG Baoyu^2,3, GAO Chengcheng^2,3, LI Xinyue¹, AN Jing^2*

(¹College Environment of Shenyang University, Shenyang 110044, China; ²Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; ³University of Chinese Academy of Sciences, Beijing 100049, China).

Online:2024-03-10 Published:2024-03-13

摘要/Abstract

摘要： 抗生素及其抗性基因可随动物粪肥施用进入农田土壤中，进而给土壤-蔬菜系统带来潜在的生态风险。为分析抗生素抗性基因在土壤及蔬菜系统中的分布特征，本研究以四环素类抗性基因（TRGs）为对象，通过温室盆栽试验研究了典型TRGs在3种可生食蔬菜（小白菜、生菜和樱桃萝卜）中的分布特征，探讨了蔬菜根系扰动对其根际土壤中TRGs多样性和丰度的影响，并分析了TRGs在蔬菜和土壤中分布特征的相关性。结果表明，受试蔬菜体内及根际土壤中均检测到TRGs和可移动元件（MGEs），检出种类和丰度依次为根际土壤>蔬菜根部>茎叶部。蔬菜根部检出的TRGs与MGEs种类和丰度高于茎叶内，其中tetD-01、tetG-01、tetT、tet(32)、IS613、tnpA-04、intI-1(clinic)检出率和丰度尤为突出。樱桃萝卜根际土壤的TRGs及MGEs比生菜、小白菜富集量大，且土壤和蔬菜系统中部分TRGs的分布与MGEs呈显著正相关。研究结果可为揭示TRGs在土壤-蔬菜系统中存在的安全风险提供数据支持。

关键词: 农田土壤, 生食蔬菜, 四环素, 抗生素抗性基因, 可移动元件

Abstract: Antibiotics and the resistance genes can enter farmland soil through fertilization of animal manure, bringing potential ecological risks to the soil-vegetable system. We examined the distribution characteristics of antibiotic resistance genes in the soil-vegetable system, with tetracycline resistance genes (TRGs) as a research object. The distribution characteristics of typical TRGs were analyzed in three kinds of raw vegetables (pakchoi, lettuce, cherry radish). The effects of vegetable root system disturbance on the diversity and abundance of TRGs in the rhizosphere soil were explored, and the correlation of TRGs between vegetable and soils were analyzed. The results showed that TRGs and mobile genetic elements (MGEs) were detected in vegetables and rhizosphere soil, with detection types and abundance following an order of rhizosphere soil>root>leaf. The types and abundances of TRGs and MGEs were higher in roots than in leaves of three tested vegetables. The detection rate and abundance of tetD-01, tetG-01, tet-T, tet(32), IS613, tnpA-04, intI-1(clinic) were prominent. TRGs and MGEs were higher in rhizosphere soil of cherry radish than that of lettuce and pakchoi. The distribution of some TRGs in soil and vegetable systems was significantly positively correlated with MGEs. Our results can provide data support for reveling the safety risks of TRGs in the soil-vegetable system.

Key words: agricultural soil, raw vegetable, tetracycline, antibiotic resistance gene, mobile genetic element

武轶, 张珣, 王宝玉, 高程程, 黎馨月, 安婧. 四环素类抗性基因在黑土农田和蔬菜中的分布特征[J]. 生态学杂志, 2024, 43(3): 758-765.

WU Yi, ZHANG Xun, WANG Baoyu, GAO Chengcheng, LI Xinyue, AN Jing. Distribution characteristics of tetracycline resistance genes in black soil farmland and vegetables.[J]. Chinese Journal of Ecology, 2024, 43(3): 758-765.

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四环素类抗性基因在黑土农田和蔬菜中的分布特征

Distribution characteristics of tetracycline resistance genes in black soil farmland and vegetables.

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