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生态学杂志 ›› 2012, Vol. 31 ›› Issue (09): 2255-2260.

• 研究报告 • 上一篇    下一篇

燕麦幼苗活性氧代谢和渗透调节物质积累对NaCl胁迫的响应

刘建新**,王金成,王瑞娟,贾海燕   

  1. (陇东学院生命科学与技术学院,甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000)
  • 出版日期:2012-09-10 发布日期:2012-09-10

Responses of active oxygen metabolism and osmotica accumulation of Avena nude L. seedlings to NaCl stress.

LIU Jian-xin**, WANG Jin-cheng, WANG Rui-Juan, JIA Hai-yan   

  1. (College of Life Science and Technology, Longdong University, Provincial Key Laboratory for Protection and Utilization of Longdong Bioresources in Gansu Province, Qingyang 745000, Gansu, China)
  • Online:2012-09-10 Published:2012-09-10

摘要: 采用营养液砂培方法,研究了不同浓度NaCl胁迫(0、50、100、150、200和250 mmol·L-1)对“定莜6号”燕麦幼苗生长、活性氧代谢和渗透调节物质含量的影响。结果表明:NaCl胁迫显著抑制燕麦幼苗的生长,抑制程度随NaCl浓度提高而增强,燕麦可耐受的最高NaCl浓度约为150 mmol·L-1;随着NaCl浓度的增加,叶片O2-产生速率、H2O2和丙二醛含量明显增加,超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶活性先升后降,过氧化氢酶活性迅速下降后逐渐升高,NaCl胁迫明显降低了谷胱甘肽含量,而抗坏血酸含量变化不大;NaCl胁迫显著提高了叶片脯氨酸含量,Na+含量随着NaCl浓度增加不断提高,K+含量和K+/Na+逐渐下降,质膜H+ATP酶活性、总可溶性蛋白、热稳定蛋白和热不稳定蛋白含量先升后降,游离氨基酸含量先降后升,可溶性糖含量呈降-升-降趋势变化;盐胁迫下活性氧代谢失调和Na+、K+平衡破坏及积累有机溶质进行渗透调节时更多能量的消耗可能是燕麦生长受抑的重要因素。

关键词: 燃烧指数, 遥感, 农田秸秆, Landsat 8

Abstract: A sand culture experiment was conducted to study the effects of NaCl stress on the seedling’s growth, active oxygen metabolism, and osmotica contents of Avena nude L. (cultivar Dingyou No. 6). Six levels (0, 50, 100, 150, 200, and 250 mmol·L-1) of NaCl were installed. NaCl stress inhibited the seedling growth significantly, and the inhibition was aggravated with increasing concentration of NaCl. The maximum concentration of NaCl that the seedlings could be in resistance to was about 150 mmol·L-1. With the increasing concentration of NaCl, the leaf O2-· production rate and H2O2 and malondialdehyde contents increased dramatically, the activities of superoxide dismutase, guaiacol peroxidase, and ascorbate peroxidase decreased after an initial increase, and the catalase activity increased gradually after a rapid decrease. The NaCl stress decreased the leaf glutathione content markedly, but the ascorbic acid content was less affected. Under NaCl stress, the leaf proline content had a significant increase. With the increasing concentration of NaCl, the leaf Na+ content increased markedly, the K+ content and K+/Na+ ratio were in adverse, the plasma membrane H+-ATPase activity and the contents of total soluble protein, heatstable protein, and heatunstable protein decreased after an initial increase, the free amino acid content decreased first and increased then, and the soluble sugar content presented a trend of decreaseincreasedecrease. All the results indicated that the imbalance of leaf active oxygen metabolism, the disturbance of leaf Na+ and K+ homeostasis, and the more consumed energy for osmotic adjustment by accumulated organic solutes under NaCl stress could be the main causes for the growth inhibition of the seedlings.

Key words: burning index, remote sensing, crop residue, Landsat 8.