欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志 ›› 2012, Vol. 31 ›› Issue (09): 2184-2190.

• 研究报告 • 上一篇    下一篇

高浓度CO2和O3对四季竹叶片膜脂过氧化及抗氧化系统的影响

庄明浩,陈双林**,李迎春,郭子武,杨清平   

  1. (中国林业科学研究院亚热带林业研究所, 浙江富阳 311400)
  • 出版日期:2012-09-10 发布日期:2012-09-10

Effects of elevated O3 and CO2 concentrations on the membrane lipid peroxidation and antioxidant systemof Oligostachyum lubricum leaves.

ZHUANG Ming-hao, CHEN Shuang-lin**, LI Ying-chun, GUO Zi-wu, YANG Qing-ping   

  1. (Research Institute of Subtropical Forestry, Chinese Forestry Academy, Fuyang 311400, Zhejiang, China
  • Online:2012-09-10 Published:2012-09-10

摘要: 以2年生四季竹为试材,利用开顶式气室(OTCs)熏蒸法模拟大气CO2和O3浓度升高情景,研究了高浓度CO2(700 μmol·mol-1)和高浓度O3(100 nmol·mol-1)及其复合作用对四季竹叶片膜脂过氧化、抗氧化酶及渗透调节物质的影响。结果表明:高浓度O3处理30 d,四季竹叶片抗氧化酶活性和渗透调节物质含量提高,清除活性氧能力增强,并未出现明显的膜脂过氧化,四季竹能耐受短期的O3胁迫。高浓度O3处理90 d,四季竹叶片抗氧化酶活性显著降低,膜脂过氧化程度加剧,膜结构破坏,发生严重的氧化损伤;高浓度CO2处理30 d,四季竹叶片抗氧化酶活性和渗透调节物质含量提高,能维持活性氧平衡,存在一定的生理适应期。而高浓度CO2处理90 d,四季竹叶片抗氧化酶活性和活性氧含量降低,可溶性糖和可溶性蛋白含量升高,有利于四季竹生长;高浓度O3和CO2复合作用下,相对于单因素高浓度O3处理,四季竹叶片能够维持较高的抗氧化酶活性和渗透调节物质含量,有效地调节活性氧产生与清除的平衡,膜脂过氧化不明显,说明高浓度CO2一定程度上可缓解高浓度O3对四季竹所造成的生理伤害。

关键词: 城乡绿地, 土壤, 水溶性有机碳, 温度敏感性, 有效磷

Abstract: By using opentop chamber, this paper studied the effects of elevated CO2(700 μmol·mol-1) and O3(100 nmol·mol-1) concentrations and their combination on the leaf membrane lipid peroxidation and antioxidant system of two years old Oligostachyum lubricum. After 30 days exposure to elevated O3 concentration, the activity of the leaf antioxidant system and the contents of the leaf osmotic adjusting substances increased, the ability of eliminating reactive oxygen production enhanced, but the malondialdehyde content had less change, suggesting that the O. lubricum could tolerate shortterm O3 stress. After 90 days exposure to elevated O3, the activity of leaf antioxidant system decreased while the leaf malondialdehyde content increased significantly, and the cell membrane structure was seriously damaged. After 30 days exposure to elevated CO2 concentration, both the activity of leaf antioxidant system and the contents of leaf osmotic adjusting substances increased, suggesting that O. lubricum had a definite physiological adaptability to high CO2 oncentrations. After 90 days exposure to elevated CO2 concentration, the activity of leaf antioxidant system and the contents of leaf reactive oxygen species decreased significantly, and the leaf soluble sugar and protein contents increased, which benefited the growth of O. lubricum. Under the effects of the combination of elevated CO2 and O3, the O. lubricum leaves could maintain high levels of antioxidant activity and osmotic adjustment substances contents to effectively regulate the balance between reactive oxygen species production and clearance, indicating that elevated CO2 could mitigate the oxidative stress in certain degree.

Key words: urbantorural green space, soil, water soluble organic carbon, temperature sensitivity, available phosphorus.