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生态学杂志 ›› 2012, Vol. 31 ›› Issue (01): 119-123.

• 研究报告 • 上一篇    下一篇

沼泽红假单胞菌对微囊藻毒素的降解

李保珍,冯佳,谢树莲**   

  1. 山西大学生命科学学院, 太原 030006
  • 出版日期:2012-01-08 发布日期:2012-01-08

Degradation of microcystin by Rhodopseudomonas palustras.

LI Bao-zhen, FENG Jia, XIE Shu-lian**   

  1. School of Life Science, Shanxi University, Taiyuan 030006, China
  • Online:2012-01-08 Published:2012-01-08

摘要: 以铜绿微囊藻为材料,通过固相萃取-高效液相色谱方法(SPE-HPLC),研究了沼泽红假单胞菌对微囊藻毒素MC-LR的降解作用。结果表明:沼泽红假单胞菌在厌氧、光照强度2000 lx、35 ℃、pH 7.0、乙酸钠为碳源、菌液初始浓度OD680为0.325和初始MC-LR为3 mg·L-1时,6 d降解率为36.5%,12 d达到最高,降解率为78.7%。此降解条件和蓝藻水华爆发的环境条件基本一致,因此该菌株在水华爆发季节消除水中的微囊藻毒素方面具有应用潜力。

关键词: 固氮树种, 人工纯林, 混交林, 土壤微生物群落, 磷脂脂肪酸, 南亚热带

Abstract: Taking Microcystis aeruginosa as test material, SPE-HPLC was adopted to study the degradation of microcystin-LR by Rhodopseudomonas palustras. Under the conditions of anaerobic, light intensity 2000 lx, 35 ℃, pH 7.0, carbon source sodium acetate, and initial concentration OD680 of the bacilli being 0.325, R. palustras could degrade 36.5% and 78.7% of the microcystin-LR with an initial concentration 3 mg·L-1 in 6 d and 12 d, respectively. These degradation conditions were basically the same environmental conditions of algal blooms, and hence, R. palustras could have practical value for removing microcystin-LR from the water body during algal blooms.

Key words: nitrogen-fixing tree species, monoculture plantation,  , mixed plantation, soil microbial community, phospholipid fatty acid, south subtropical China.