Application of PCR-denaturing gradient gel electrophoresis (PCR-GGE) in environmental microbial ecology

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Application of PCR-denaturing gradient gel electrophoresis (PCR-GGE) in environmental microbial ecology

SHAN Guobin^1,2; JIN Wenbiao³; LIN Jikan^1,4; XING Xinhui²

¹Baolimei Chemical Engineering Company Limited, Dongguan 523581, China;²Institute of Biochemical Engineering, Department of Chemical Engineering,Tsinghua University, Beijing 100084, China;³College of Urban and Civil Engineering, Shenzhen Graduate School of Harbin Institute of Technology, Shenzhen 518055, China;⁴Bio-Treat Technology Company Limited, Dongguan 523581, China

Received:2006-01-20 Revised:2006-06-01 Online:2006-10-10 Published:2006-10-10

Abstract

Abstract: PCR-denaturing gradient gel electrophoresis (PCR-DGGE) has the advantages of high reliability, good repetition and easy operation, and been widely used in environmental ecology to analyze the diversity and dynamics of microbial communities, and to monitor functional bacteria. This paper introduced the principles and methods of PCR-DGGE, reviewed its application in environmental ecological study , and analyzed its potential application prospects and limitations.

Key words: Element sulphur, Vegetable soil, Nitrate, Nitrification inhibitor

SHAN Guobin^1,2; JIN Wenbiao³; LIN Jikan^1,4; XING Xinhui² . Application of PCR-denaturing gradient gel electrophoresis (PCR-GGE) in environmental microbial ecology[J]. cje.

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Application of PCR-denaturing gradient gel electrophoresis (PCR-GGE) in environmental microbial ecology

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