Abstract: This paper introduced the calculation methods of ecological footprint,and reviewed the research advances in ecological footprint at home and abroad,with the focus on energy footprint calculation.At present,the study of ecological footprint at abroad was centralized on the long time series calculation and analysis and the improvement of calculation method,and based on these,an improved ecological footprint method,“actual land demand” method,was submitted.In China,the work was more defined to the case studies of ecological footprint.In this review,the advantages and disadvantages of ecological footprint methods were also discussed,and the future research directions of ecological footprint were put forward.

Key words: Bacillus subtilis, Transgenic plant, Phytase, Phytate, Phosphorus uptake and utilization, Tobacco