Arsenate reduction characteristics and diversity analysis of arsenic-reducing bacteria enriched from an arsenic contaminated soil.

cje

• Articles • Previous Articles Next Articles

Arsenate reduction characteristics and diversity analysis of arsenic-reducing bacteria enriched from an arsenic contaminated soil.

ZHANG Xue-xia; JIA Yong-feng;CHEN Liang;WANG Xin;XU Li-ying;ZHANG Xu-dong

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China

Received:2008-05-13 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10

Abstract

Abstract: Arsenic (As)-resistance bacteria were enriched from an As-contaminated soil and cultured in anaerobic environment, aimed to study their arsenate reduction capability and microbial diversity. Within 21 h incubation, arsenate was completely reduced to arsenite; after 72 h incubation, yellow precipitates formed; and after 150 h incubation, about 65% of soluble arsenic was removed. SEM-EDS and XRD analyses revealed that the yellow precipitates were mainly of three kinds of AsS crystalloid. The microbial diversity in the culture was investigated by 16S rDNA-dependent molecular phylogeny, and a near-full-length 16S rDNA gene clone library was constructed. 197 clones were analyzed by using RFLP, and a total of 72 OTUs were obtained, among which, six OTUs occupied 51% of the volume. One clone from each of the six OTUs was selected for sequencing, and it was indicated that the enriched As-reducing bacteria belonged to Caloramator, Clostridium, and Bacillus.

Key words: Biological invasion, Approach and damage, Prevention measures

ZHANG Xue-xia; JIA Yong-feng;CHEN Liang;WANG Xin;XU Li-ying;ZHANG Xu-dong. Arsenate reduction characteristics and diversity analysis of arsenic-reducing bacteria enriched from an arsenic contaminated soil.[J]. cje.

[1]	WU Hao, JIA Shao-qi, ZHU Ya-xing. Effects of species diversity and environmental factors on the stability of the invasive Alternanthera philoxeroides communities [J]. Chinese Journal of Ecology, 2022, 41(1): 33-41.
[2]	. Distribution patterns and influencing factors of naturalized plant species in Heilongjiang, Jilin, Liaoning, and Inner Mongolia. [J]. cje, 2020, 39(5): 1492-1500.
[3]	XIONG Yun-qi, ZHAO Cai-yun. Phenotypic plasticity and the successful invasion of alien plants. [J]. Chinese Journal of Ecology, 2020, 39(11): 3853-2864.
[4]	JIN Shu-rui, LUO Du, ZHANG Hai-tao, MU Xi-dong, HU Yin-chang. Responses of the invasive golden apple snails (Pomacea canaliculata) at different life-history stages to acidic stress. [J]. cje, 2019, 38(5): 1426-1432.
[5]	ZHANG Jia-en, GUO Jing, ZHAO Ben-liang, QIN Zhong. Research prospect of all-round controlling technology system constructing and resource-based utilization of Pomacea canaliculata in paddy fields. [J]. cje, 2019, 38(12): 3831-3838.
[6]	XU Guang-yao, LI Hong-yuan, MO Xun-qiang, MENG Wei-qing. Restraining factors on the effectiveness of invasive plants management and control. [J]. cje, 2019, 38(10): 3169-3176.
[7]	FENG Jian-xiang, HUANG Qian, CHEN Hui, SHEN Qian-qian, HUANG Jian-rong, LI Zu-fu. Effects ofSpartina alterniflora invasion on benthic faunal community in saltmarsh and mangrove wetland. [J]. cje, 2018, 37(3): 943-951.
[8]	XU Guang-yao, LI Hong-yuan, MENG Wei-qing, MO Xun-qiang. Discrimination of “secondary invasion” and plant secondary invasion. [J]. cje, 2018, 37(12): 3780-3786.
[9]	ZHANG Can-yu1,2, XU Meng1, FANG Miao1,2, LUO Du1, GU Dang-en1, MU Xi-dong1, WEI Hui1, HU Yin-chang1. Variation of egg spot traits of Pomacea canaliculata*, an invasive species across latitudes. [J]. cje, 2017, 36(6): 1628-1636.
[10]	GAO Yu, LIU Jian-yi, ZHANG Ting-ting, FENG Guang-peng, ZHANG Tao, YANG Gang, ZHUANG Ping*. Escaped aquacultural species promoted the alien species invasion in the Yangtze River: A case study of sturgeons. [J]. cje, 2017, 36(6): 1739-1745.
[11]	XIA Han-ping1**, WANG Ming-zhu2, XU Li-yu2. Is vetiver grass planted in China an invasive alien species and becoming a weed? [J]. cje, 2015, 34(8): 2327-2332.
[12]	GUO Jing1, ZHANG Jia-en1,2,3*. Status, problems and countermeasures in biological control of Pomacea canaliculata*.  [J]. cje, 2015, 34(10): 2943-2950.
[13]	GUO Jing1, ZHANG Jia-en1,2,3*. Research progress on invasion mechanisms of Pomacea canaliculata*. [J]. cje, 2014, 33(3): 806-815.
[14]	ZHAO Xiang-jian1, LIU Xiao-yan1, GONG Lu1, DENG Zhen-zhen1, BAI Jia-de2, LI Jun-sheng1, ZHAO Cai-yun1*. Control of Spartina alterniflora* by integrated technique of mowing plus shading. [J]. cje, 2014, 33(10): 2714-2719.
[15]	JI Hai-ting, XIE Dong, ZHOU Heng-jie, AN Shu-qing**. Effects of leaf extracts from two kinds of submerged macrophytes on the growth and community composition of epiphytic algae living on native macrophyte species. [J]. cje, 2013, 32(9): 2298-2306.

Arsenate reduction characteristics and diversity analysis of arsenic-reducing bacteria enriched from an arsenic contaminated soil.

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments