Abstract: A field experiment was conducted to study the leaf C, N and P stoichiometry of 2-year-old Phyllostachys iridescens individuals under long-term application of bio-organic fertilizer, compound fertilizer and rapeseed cake fertilizer, aimed to provide scientific basis for the rational supplement of soil nutrients in bamboo-shoot-use P. iridescens stand. In all treatments, the leaf C, N and P concentrations were 514.26-582.77 mg·g^-1, 18.25-30.20 mg·g^-1, and 1.20-1.75 mg·g^-1,respectively. Fertilization increased the leaf C, N, and P concentrations significantly. The leaf C concentration was the highest under the application of rapeseed cake fertilizer, and the leaf N and P concentrations were the highest under the application of bio-organic fertilizer. The leaf C：N and C：P ratios in all treatments were 18.71-35.02 and 304.41-458.52, respectively, and fertilization decreased these ratios significantly, being most obvious under the application of bio-organic fertilizer. The leaf N：P ratio ranged from 15.28 to 17.12, and no significant difference was observed among all treatments. There was a significant positive correlation between leaf N and P, no correlation between leaf N and N：P, and a significant negative correlation between leaf P and N：P.

Key words: Brachionus calyciflorus, Strain, Temperature, Food concentration, Life history parameter